通过导入黑腹果蝇进行物种形成的遗传分析。
Genetic analysis of speciation by means of introgression into Drosophila melanogaster.
作者信息
Sawamura K, Davis A W, Wu C I
机构信息
Department of Ecology and Evolution, University of Chicago, 1101 East 57th Street, Chicago, IL 60637, USA.
出版信息
Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2652-5. doi: 10.1073/pnas.050558597.
In the last decade, the genetic basis of reproductive isolation has been shown to be surprisingly polygenic, and yet even the most efficient system currently in use could lend itself to molecular analysis only in highly selected cases. By extending the recent discovery of fertility rescue between Drosophila melanogaster and Drosophila simulans, we show that this hybridization can permit systematic and precise delineation of the genetic and molecular basis of speciation. In a region of 5% of the D. simulans genome introgressed into D. melanogaster, we discover at least six genes of hybrid male sterility and none for female sterility by deficiency mapping. A single case of hybrid inviability has been tracked down to a 3-Kb element that was inserted into the Cyclin E locus during species hybridization. The extent of interspecific genetic divergence underlying hybrid male sterility, especially in contrast with the low degree of inviability and female sterility, is far greater than expected from previous studies.
在过去十年中，生殖隔离的遗传基础已被证明惊人地具有多基因性，然而，即使是目前使用的最有效的系统，也只有在经过高度筛选的情况下才能用于分子分析。通过扩展最近在黑腹果蝇和拟暗果蝇之间发现的育性拯救现象，我们表明这种杂交能够对物种形成的遗传和分子基础进行系统而精确的描绘。在导入到黑腹果蝇中的拟暗果蝇基因组5%的区域内，我们通过缺失定位发现了至少六个导致杂交雄性不育的基因，而未发现导致雌性不育的基因。一例杂交 inviability（此处可能有误，推测是 inviability，即杂交不活）被追踪到一个在物种杂交过程中插入到细胞周期蛋白E基因座的3千碱基元件。杂交雄性不育背后的种间遗传差异程度，尤其是与低程度的杂交不活和雌性不育相比，远远大于先前研究所预期的。
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