Buliakova N V, Azarova V S
Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow.
Tsitologiia. 2000;42(1):27-31.
It is known that a low-energy laser radiation can cause reflex suppression of immunity. The present experiments were designed to determine the plastic activity of allogenic muscle tissue in different conditions of a previous action of laser rays. The cross homotransplantation of gastrocnemius muscles was carried out between intact rats, or between rats in which 14 days before transplantation each hind leg was subjected to low-energy He-Ne laser radiation in dose of 7.5-9 J/cm2 (10 procedures, the duration of each exposure was equal to 5 min), or between intact and radiated rats. It was shown that the donor muscle tissue survived longer when a nonradiated muscle was transplanted into the radiated muscle bed. The axons grew into the donor muscle tissue. More allogenic muscle tissue was involved in contractile reaction when stimulation was carried out via the nerve. Laser radiation of a homotransplant alone, or that of a homotransplant and a muscle bed in the recipient was less effective. So, He-Ne laser radiation of the area of a planned allotransplantation decreased the transplant immunity response and favoured a longer development of allogenic muscle tissue. The viability of donor muscle tissue therewith increased, if the muscle allograft had not been subjected to a previous laser radiation.
众所周知，低能量激光辐射可引起免疫的反射性抑制。本实验旨在确定在激光射线先前作用的不同条件下同种异体肌肉组织的可塑性活动。在完整大鼠之间、或在移植前14天每条后腿接受剂量为7.5 - 9 J/cm²的低能量氦氖激光辐射（10次，每次照射持续时间为5分钟）的大鼠之间、或在完整大鼠与接受辐射的大鼠之间进行腓肠肌的交叉同种移植。结果表明，当将未辐射的肌肉移植到接受辐射的肌肉床时，供体肌肉组织存活时间更长。轴突长入供体肌肉组织。当通过神经进行刺激时，更多的同种异体肌肉组织参与收缩反应。单独对同种移植组织进行激光辐射，或对受体中的同种移植组织和肌肉床进行激光辐射效果较差。因此，对计划进行同种异体移植的区域进行氦氖激光辐射可降低移植免疫反应，并有利于同种异体肌肉组织的更长时间发育。如果肌肉同种异体移植未预先接受激光辐射，则供体肌肉组织的活力会随之增加。
