Rhiouani H, Settaf A, Lyoussi B, Cherrah Y, Lacaille-Dubois M A, Hassar M
Department of Biology, Faculty of Science D.M., Fez, Morocco.
Therapie. 1999 Nov-Dec;54(6):735-9.
Experiments were performed on male and female spontaneously hypertensive rats weighing 310-340 g (10 animals per group). The oral administration of 200 mg/kg/day of saponins from Herniaria glabra for 30 days, resulted in a significant decrease in blood pressure in hypertensive rats. The systolic and diastolic blood pressure decreased significantly and respectively from 187.60 +/- 20.63/119.00 +/- 7.09 mmHg at day 0 (D0) to 141.60 +/- 7.51/90.40 +/- 7.68 mmHg at day 30 (D30), p < 0.001 (vs. 186.30 +/- 11.27/114.10 +/- 12.00 mm Hg at D0 to 154.50 +/- 6.38/132.3 +/- 7.68 mmHg at D30 in furosemide-treated group, p < 0.001). Control animals receiving placebo did not show any significant variation in the mean arterial pressure. The effect of saponins of Herniaria glabra on renal function was evaluated in spontaneously hypertensive rats using clearance techniques. Glomerular filtration rate was constant in the control rats and increased significantly in the hypertensive rats after saponins treatment (5.55 +/- 0.32 vs. 6.03 +/- 0.43 ml.min-1.kg-1 in the control (C) and saponins (S) groups, respectively, p < 0.05). Saponins administration provoked an increase in urinary flow (59.38 +/- 5.85 ml.kg-1.24 h-1 vs. 36.92 +/- 5.17 ml.kg-1.24 h-1, p < 0.001). Saponins also increased potassium excretion (6.89 +/- 0.81 mmol.kg-1.24 h-1 vs. 5.40 +/- 0.51 mmol.kg-1.24 h-1, p < 0.001) and sodium excretion (10.74 +/- 1.21 mmol.kg-1.24 h-1 vs. 7.25 +/- 0.54 mmol.kg-1.24 h-1, p < 0.001) as well as chloride excretion (13.59 +/- 1.04 mmol. kg-1.24 h-1 vs. 9.67 +/- 0.77 mmol.kg-1.24 h-1, p < 0.001). It is concluded that chronic oral administration of saponins from Herniaria glabra decreased the arterial blood pressure and affected salt and water transport in renal tubules.
对体重310 - 340克的雄性和雌性自发性高血压大鼠进行实验（每组10只动物）。每天口服200毫克/千克光果脐草皂苷，持续30天，可使高血压大鼠的血压显著降低。收缩压和舒张压分别从第0天（D0）的187.60±20.63/119.00±7.09毫米汞柱显著降至第30天（D30）的141.60±7.51/90.40±7.68毫米汞柱，p < 0.001（呋塞米治疗组从D0的186.30±11.27/114.10±12.00毫米汞柱降至D30的154.50±6.38/132.3±7.68毫米汞柱，p < 0.001）。接受安慰剂的对照动物平均动脉压未显示任何显著变化。采用清除技术在自发性高血压大鼠中评估光果脐草皂苷对肾功能的影响。对照大鼠的肾小球滤过率保持恒定，皂苷治疗后高血压大鼠的肾小球滤过率显著增加（对照组（C）和皂苷组（S）分别为5.55±0.32与6.03±0.43毫升·分钟⁻¹·千克⁻¹，p < 0.05）。给予皂苷可使尿流量增加（59.38±5.85毫升·千克⁻¹·24小时⁻¹对36.92±5.17毫升·千克⁻¹·24小时⁻¹，p < 0.001）。皂苷还增加了钾排泄（6.89±0.81毫摩尔·千克⁻¹·24小时⁻¹对5.40±0.51毫摩尔·千克⁻¹·24小时⁻¹，p < 0.001）、钠排泄（10.74±1.21毫摩尔·千克⁻¹·24小时⁻¹对7.25±0.54毫摩尔·千克⁻¹·24小时⁻¹，p < 0.001）以及氯排泄（13.59±1.04毫摩尔·千克⁻¹·24小时⁻¹对9.67±0.77毫摩尔·千克⁻¹·24小时⁻¹，p < 0.001）。结论是，长期口服光果脐草皂苷可降低动脉血压并影响肾小管中的盐和水转运。
