Zeggwagh N-A, Farid O, Michel J B, Eddouks M
Moulay Ismail University, Errachidia, Morocco.
Methods Find Exp Clin Pharmacol. 2008 Jun;30(5):375-81. doi: 10.1358/mf.2008.30.5.1186081.
This study aimed to evaluate the hypotensive activity of Artemisia herba alba aqueous extract (AHAE) in spontaneously hypertensive rats (SHR). AHAE was lyophilized and administered daily at a dose of 150 mg/kg for 20 days. AHAE administration produced a significant reduction in systolic blood pressure after 8 days of oral administration (P < 0.01), and a sustained reduction was observed at the end of treatment (P < 0.01). Heart rate remained unchanged during the 20 days of oral AHAE administration. In addition, AHAE administration produced a significant increase in urinary output (P < 0.01) and glomerular filtration rate (P < 0.01) on day 8 of treatment. Urinary electrolyte excretion was also modified during the 20 days of AHAE administration, and a significant increase in urinary sodium and potassium excretion was observed from day 4 (P < 0.01) to day 20 (P < 0.001). However, urinary chloride excretion was increased from day 8 (P < 0.01) to the end of treatment (P < 0.001). The hypotensive effect appeared to be independent of the renin-angiotensin system since AHAE did not affect plasma angiotensin-converting enzyme or renin activities (P > 0.05) after 20 days of oral administration. We conclude that AHAE possesses antihypertensive activity in SHR and that the underlying mechanism appears to involve, at least in part, an increase in urine and electrolyte output.
本研究旨在评估白蒿水提取物（AHAE）对自发性高血压大鼠（SHR）的降压活性。将AHAE冻干，以150 mg/kg的剂量每日给药，持续20天。口服给药8天后，AHAE可使收缩压显著降低（P < 0.01），治疗结束时观察到收缩压持续降低（P < 0.01）。在口服AHAE的20天内，心率保持不变。此外，治疗第8天时，AHAE可使尿量（P < 0.01）和肾小球滤过率显著增加（P < 0.01）。在AHAE给药的20天内，尿电解质排泄也发生了改变，从第4天（P < 0.01）到第20天（P < 0.001），尿钠和钾排泄显著增加。然而，尿氯排泄从第8天（P < 0.01）到治疗结束时增加（P < 0.001）。口服给药20天后，AHAE对血浆血管紧张素转换酶或肾素活性无影响（P > 0.05），因此其降压作用似乎与肾素-血管紧张素系统无关。我们得出结论，AHAE对SHR具有降压活性，其潜在机制似乎至少部分涉及尿液和电解质排出量的增加。
