Kaur Gurpreet, Jaggi Amteshwar Singh, Singh Nirmal
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India.
J Brachial Plex Peripher Nerve Inj. 2010 Jan 25;5:3. doi: 10.1186/1749-7221-5-3.
The present study was designed to investigate the ameliorative potential of Ocimum sanctum and its saponin rich fraction in vincristine-induced peripheral neuropathic pain in rats. Peripheral neuropathy was induced in rats by administration of vincristine sulfate (50 mug/kg i.p.) for 10 consecutive days. The mechanical hyperalgesia, cold allodynia, paw heat hyperalgesia and cold tail hyperalgesia were assessed by performing the pinprick, acetone, hot plate and cold tail immersion tests, respectively. Biochemically, the tissue thio-barbituric acid reactive species (TBARS), super-oxide anion content (markers of oxidative stress) and total calcium levels were measured. Vincristine administration was associated with the development of mechanical hyperalgesia, cold allodynia, heat and cold hyperalgesia. Furthermore, vincristine administration was also associated with an increase in oxidative stress and calcium levels. However, administration of Ocimum sanctum (100 and 200 mg/kg p.o.) and its saponin rich fraction (100 and 200 mg/kg p.o.) for 14 days significantly attenuated vincristine-induced neuropathic pain along with decrease in oxidative stress and calcium levels. It may be concluded that Ocimum sanctum has ameliorative potential in attenuating chemotherapy induced-painful neuropathic state, which may be attributed to decrease in oxidative stress and calcium levels. Furthermore, saponin rich fraction of Ocimum sanctum may be responsible for its noted beneficial effect in neuropathic pain in rats.
本研究旨在探讨神圣罗勒及其富含皂苷的组分对长春新碱诱导的大鼠周围神经性疼痛的改善潜力。通过连续10天腹腔注射硫酸长春新碱（50μg/kg）诱导大鼠周围神经病变。分别通过针刺、丙酮、热板和冷尾浸没法评估机械性痛觉过敏、冷觉异常性疼痛、爪部热痛觉过敏和冷尾热痛觉过敏。生化方面，检测组织硫代巴比妥酸反应性物质（TBARS）、超氧阴离子含量（氧化应激标志物）和总钙水平。给予长春新碱与机械性痛觉过敏、冷觉异常性疼痛、热痛觉过敏和冷痛觉过敏的发生有关。此外，给予长春新碱还与氧化应激和钙水平升高有关。然而，连续14天给予神圣罗勒（100和200mg/kg口服）及其富含皂苷的组分（100和200mg/kg口服）可显著减轻长春新碱诱导的神经性疼痛，并降低氧化应激和钙水平。可以得出结论，神圣罗勒在减轻化疗诱导的疼痛性神经病变状态方面具有改善潜力，这可能归因于氧化应激和钙水平的降低。此外，神圣罗勒富含皂苷的组分可能是其对大鼠神经性疼痛具有显著有益作用的原因。
