Rausch R N, Crawshaw L I, Wallace H L
Department of Biology, Organismal Section, Portland State University, Portland 97207, Oregon.
Am J Physiol Regul Integr Comp Physiol. 2000 Mar;278(3):R545-55. doi: 10.1152/ajpregu.2000.278.3.R545.
Effects of hypoxia, anoxia, and endogenous ethanol (EtOH) on selected temperature (T(sel)) and activity in goldfish were evaluated. Blood and brain EtOH concentrations ([EtOH]) and brain oxygen partial pressure (PO(2)) were quantified at crucial ambient oxygen pressures. Below a threshold value near 31 Torr, T(sel) decreased as a function of environmental PO(2). T(sel) of 15 degrees C-acclimated fish was approximately 10 degrees C at the onset of anoxia and changed little over 2 h. Activity showed a similar response pattern. Brain [EtOH] was significantly elevated above control levels after 1 h anoxia. In normoxic water, T(sel) remained different in previously anoxic and normoxic control fish for approximately 20 min. Blood [EtOH] of previously anoxic fish remained significantly elevated ([EtOH] >4.0 micromol/g blood), and activity was significantly depressed at 20 min. Brain PO(2) reached normal levels in <3 min. We conclude that [EtOH] (brain or blood) and brain PO(2) are not proximal causes of either behavioral anapyrexia (hypothermia) or inactivity in goldfish exposed to oxygen-depleted environments.
评估了缺氧、无氧和内源性乙醇（EtOH）对金鱼选定温度（T(sel)）和活动的影响。在关键环境氧压下对血液和大脑中的乙醇浓度（[EtOH]）以及大脑氧分压（PO(2)）进行了定量分析。在接近31托的阈值以下，T(sel)随环境PO(2)的降低而下降。15℃驯化的鱼在无氧开始时的T(sel)约为10℃，并且在2小时内变化不大。活动表现出类似的反应模式。无氧1小时后，大脑[EtOH]显著高于对照水平。在常氧水中，先前无氧和常氧对照鱼的T(sel)在约20分钟内仍存在差异。先前无氧鱼的血液[EtOH]仍显著升高（[EtOH]>4.0微摩尔/克血液），并且在20分钟时活动显著降低。大脑PO(2)在不到3分钟内恢复到正常水平。我们得出结论，在暴露于缺氧环境的金鱼中，[EtOH]（大脑或血液）和大脑PO(2)不是行为性体温过低（体温过低）或活动减少的直接原因。
