O'Connor C S, Crawshaw L I, Bedichek R C, Crabbe J C
Department of Biology, Portland State University, OR 97207.
Pharmacol Biochem Behav. 1988 Feb;29(2):243-8. doi: 10.1016/0091-3057(88)90152-9.
The effect of ethanol on behavioral thermoregulation in the goldfish, Carassius auratus, was studied by adding ethanol to a horizontal aquatic temperature gradient which allowed each fish to select its preferred temperature within a range of about 9 degrees C to 33 degrees C. Alternating exposure to 1.0% (v/v) ethanol and water showed that fish (10 to 15 g) responded to ethanol by selecting lower temperatures. Onset and disappearance of the effect occurred within 10 min of exposure to or removal from ethanol. Fish exposed to 1.0% ethanol for 3 hr did not show acute tolerance. When fish were exposed to increasing concentrations of ethanol from 0.0% to 1.7%, the lowest concentration to elicit a response was 0.5% ethanol. The magnitude of the response plateaued at 0.7% ethanol. At this concentration and above, selected temperatures remained about 2 degrees C below temperatures selected by controls. Because thermoregulatory responses of fish are behavioral and relatively easy to observe and quantify, goldfish offer a useful model for the study of ethanol effects on central nervous system control of thermoregulation. Ethanol produces a prompt, stable, and reproducible depression of selected temperature by lowering the thermoregulatory set point in the goldfish.
通过在水平水生温度梯度中添加乙醇，研究乙醇对金鱼（Carassius auratus）行为性体温调节的影响，该温度梯度使每条鱼能够在约9摄氏度至33摄氏度的范围内选择其偏好的温度。交替暴露于1.0%（v/v）乙醇和水中表明，体重为10至15克的鱼通过选择更低的温度对乙醇做出反应。这种影响的开始和消失在暴露于乙醇或从乙醇中移除后的10分钟内发生。暴露于1.0%乙醇3小时的鱼未表现出急性耐受性。当鱼暴露于从0.0%至1.7%逐渐增加的乙醇浓度时，引发反应的最低浓度为0.5%乙醇。反应幅度在0.7%乙醇时达到平稳。在此浓度及以上，所选温度比对照组所选温度低约2摄氏度。由于鱼的体温调节反应是行为性的，相对容易观察和量化，金鱼为研究乙醇对体温调节中枢神经系统控制的影响提供了一个有用的模型。乙醇通过降低金鱼的体温调节设定点，对所选温度产生迅速、稳定且可重复的降低作用。
