Wang H, Ng T B, Ooi V E, Liu W K
Department of Biology, The Chinese University of Hong Kong, Shatin, NT.
Int J Biochem Cell Biol. 2000 Mar;32(3):365-72. doi: 10.1016/s1357-2725(99)00130-2.
The effects of lectins with different carbohydrate-binding specificities on human hepatoma (H3B), human choriocarcinoma (JAr), mouse melanoma (B16) and rat osteosarcoma (ROS) cell lines were investigated. Cell viability was estimated by uptake of crystal violet. Wheat germ lectin was the lectin with the most deleterious effect on the viability of H3B, JAr and ROS cell lines. The cytotoxicity of lectins with similar sugar-binding specificity to wheat germ lectin, including Maackia amurensis lectin and Solanum tuberosum lectin, was weaker than that of wheat germ lectin. N-acetylgalactosamine-and galactose-binding Tricholoma mongolicum lectin ranked third, after wheat germ lectin and Maackia amurensis lectin, with regard to its effect on H3B, and ranked, together with Maackia amurensis lectin, as the lectins with the second most pronounced effects on ROS. However, the cytotoxic effects of Tricholoma mongolicum lectin on JAr were much weaker than those of Maackia amurensis lectin, Solanum tuberosum lectin and Anguilla anguilla lectin. Artocarpus integrifolia lectin, Lens culinaris lectin and Anguilla anguilla lectin possessed milder cytotoxicity than the remaining lectins. which were approximately equipotent. The mannose-binding Narcissus pseudonarcissus and Lens culinaris lectins were only weakly cytotoxic, the exception being a stronger effect on H3B. The N-acetylgalactosamine-binding Glycine max lectin and methylgalactose-binding Artocarpus integrifolia lectin similarly exhibited low cytotoxicity. It can thus be concluded that in general the ranking was wheat germ lectin > Maackia amurensis lectin approximately Trichloma mongolicum lectins > other aforementioned lectins in cytotoxicity. A particular lectin may manifest more conspicuous toxicity on certain cell lines and less on others.
研究了具有不同碳水化合物结合特异性的凝集素对人肝癌细胞系（H3B）、人绒毛膜癌细胞系（JAr）、小鼠黑色素瘤细胞系（B16）和大鼠骨肉瘤细胞系（ROS）的影响。通过结晶紫摄取来评估细胞活力。麦胚凝集素对H3B、JAr和ROS细胞系的活力具有最有害的影响。与麦胚凝集素具有相似糖结合特异性的凝集素，包括山槐凝集素和马铃薯凝集素，其细胞毒性比麦胚凝集素弱。就对H3B的影响而言，结合N - 乙酰半乳糖胺和半乳糖的蒙古口蘑凝集素仅次于麦胚凝集素和山槐凝集素，排第三位；就对ROS的影响而言，它与山槐凝集素并列，是第二显著的凝集素。然而，蒙古口蘑凝集素对JAr的细胞毒性比山槐凝集素、马铃薯凝集素和鳗鲡凝集素弱得多。波罗蜜凝集素、小扁豆凝集素和鳗鲡凝集素的细胞毒性比其余凝集素温和，它们的细胞毒性大致相当。结合甘露糖的水仙凝集素和小扁豆凝集素细胞毒性较弱，对H3B的影响是个例外，其作用较强。结合N - 乙酰半乳糖胺的大豆凝集素和结合甲基半乳糖的波罗蜜凝集素同样表现出低细胞毒性。因此可以得出结论，一般来说，细胞毒性的排序为：麦胚凝集素＞山槐凝集素≈蒙古口蘑凝集素＞其他上述凝集素。一种特定的凝集素可能对某些细胞系表现出更明显的毒性，而对其他细胞系则较弱。
