伯氏疏螺旋体VlsE免疫显性恒定区在三种宿主物种中的表位作图
Epitope mapping of the immunodominant invariable region of Borrelia burgdorferi VlsE in three host species.
作者信息
Liang F T, Philipp M T
机构信息
Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433, USA.
出版信息
Infect Immun. 2000 Apr;68(4):2349-52. doi: 10.1128/IAI.68.4.2349-2352.2000.
Abstract
VlsE, the variable surface antigen of Borrelia burgdorferi, contains a 26-amino-acid-long immunodominant invariable region, IR(6). In the present study, three overlapping 14-mer peptides reproducing the sequence of IR(6) were used as peptide-based enzyme-linked immunosorbent assay antigens to map this invariable region in infected monkeys, mice, and human Lyme disease patients. Antibodies of the two primate species appeared to recognize IR(6) as a single antigenic determinant, while mouse antibodies recognized multiple epitopes within this region.
摘要
伯氏疏螺旋体的可变表面抗原VlsE含有一个26个氨基酸长的免疫显性恒定区IR(6)。在本研究中，三种重叠的14肽再现了IR(6)的序列，用作基于肽的酶联免疫吸附测定抗原，以在受感染的猴子、小鼠和人类莱姆病患者中定位这个恒定区。两种灵长类动物的抗体似乎将IR(6)识别为单一抗原决定簇，而小鼠抗体识别该区域内的多个表位。
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本文引用的文献
1
Analysis of antibody response to invariable regions of VlsE, the variable surface antigen of Borrelia burgdorferi.针对伯氏疏螺旋体可变表面抗原VlsE恒定区的抗体反应分析。
Infect Immun. 1999 Dec;67(12):6702-6. doi: 10.1128/IAI.67.12.6702-6706.1999.
2
Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi vlsE.通过基于伯氏疏螺旋体vlsE免疫显性保守区的肽进行酶联免疫吸附测定对莱姆病进行灵敏且特异的血清学诊断。
J Clin Microbiol. 1999 Dec;37(12):3990-6. doi: 10.1128/JCM.37.12.3990-3996.1999.
3
An immunodominant conserved region within the variable domain of VlsE, the variable surface antigen of Borrelia burgdorferi.伯氏疏螺旋体可变表面抗原VlsE可变区内的一个免疫显性保守区域。
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4
Genetic and immunological analyses of Vls (VMP-like sequences) of Borrelia burgdorferi.伯氏疏螺旋体Vls（VMP样序列）的遗传学和免疫学分析。
Microb Pathog. 1998 Mar;24(3):155-66. doi: 10.1006/mpat.1997.0183.
5
The outer surface protein A (OspA) vaccine against Lyme disease: efficacy in the rhesus monkey.抗莱姆病的外表面蛋白A（OspA）疫苗：在恒河猴中的疗效
Vaccine. 1997 Dec;15(17-18):1872-87. doi: 10.1016/s0264-410x(97)00133-3.
6
Antigenic variation in Lyme disease borreliae by promiscuous recombination of VMP-like sequence cassettes.莱姆病疏螺旋体通过VMP样序列盒的杂乱重组实现抗原变异。
Cell. 1997 Apr 18;89(2):275-85. doi: 10.1016/s0092-8674(00)80206-8.
7
Early and early disseminated phases of Lyme disease in the rhesus monkey: a model for infection in humans.恒河猴莱姆病的早期和早期播散阶段：人类感染的模型
Infect Immun. 1993 Jul;61(7):3047-59. doi: 10.1128/iai.61.7.3047-3059.1993.
8
Saliva of the Lyme disease vector, Ixodes dammini, blocks cell activation by a nonprostaglandin E2-dependent mechanism.莱姆病传播媒介达氏硬蜱的唾液通过一种非前列腺素E2依赖机制阻断细胞活化。
J Exp Med. 1994 Sep 1;180(3):1077-85. doi: 10.1084/jem.180.3.1077.
9
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