Yamamoto K, Maruyama I N, Osawa T
Laboratory of Molecular Medicine, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Japan.
J Biochem. 2000 Jan;127(1):137-42. doi: 10.1093/oxfordjournals.jbchem.a022575.
Bauhinia purpurea lectin (BPA) is one of the beta-galactose-binding leguminous lectins. Leguminous lectins contain a long metal-binding loop, part of which determines their carbohydrate-binding specificities. Random mutations were introduced into a portion of the cDNA coding BPA that corresponds to the carbohydrate-binding loop of the lectin. An library of the mutant lectin expressed on the surface of lambda foo phages was screened by the panning method. Several phage clones with an affinity for mannose or N-acetylglucosamine were isolated. These results indicate the possibility of making artificial lectins (so-called "cyborg lectins") with distinct and desired carbohydrate-binding specificities.
紫羊蹄甲凝集素（BPA）是一种结合β-半乳糖的豆科凝集素。豆科凝集素含有一个长的金属结合环，其中一部分决定了它们的碳水化合物结合特异性。对编码BPA的cDNA中与凝集素碳水化合物结合环相对应的部分进行随机突变。通过淘选法筛选在λ噬菌体表面表达的突变凝集素文库。分离出了几个对甘露糖或N-乙酰葡糖胺有亲和力的噬菌体克隆。这些结果表明制造具有独特和所需碳水化合物结合特异性的人工凝集素（所谓的“半机械人凝集素”）是有可能的。
