Yamamoto K, Konami Y, Osawa T, Irimura T
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo.
J Biochem. 1992 Jan;111(1):87-90. doi: 10.1093/oxfordjournals.jbchem.a123724.
A chimeric lectin gene was constructed by using a cDNA clone coding the Bauhinia purpurea lectin (BPA) in which a part of the metal-binding region was replaced by the corresponding region of the mannose-binding Lens culinaris lectin (LCA). The chimeric lectin expressed in Escherichia coli was found to bind alpha mannosyl-bovine serum albumin (BSA) and this binding was inhibited by mannose.
通过使用编码紫羊蹄甲凝集素（BPA）的cDNA克隆构建了一个嵌合凝集素基因，其中金属结合区域的一部分被甘露糖结合的兵豆凝集素（LCA）的相应区域所取代。发现在大肠杆菌中表达的嵌合凝集素能结合α-甘露糖基牛血清白蛋白（BSA），并且这种结合被甘露糖抑制。
