Nakao M, Yokota K, Oguma K, Takai K
Department of Nursing, Faculty of Health and Welfare Science, Okayama Prefectural University.
Kansenshogaku Zasshi. 2000 Feb;74(2):120-7. doi: 10.11150/kansenshogakuzasshi1970.74.120.
The bacteriocidal effect of electrolysed functioning water against Helicobacter pylori was examined by both the culture method (viable count on agar plate) and the bacterial ATP content analysis (biolumminescence assay). The culture method revealed the water's reduced bacteriocidal effect on organic substances such as horse serum and bovine serum albumin. The bacterial ATP content determined by the treatment with lysis solution correlated with the bacterial cell count. The ATP of bacteria treated with electrolysed functioning water, benzalkonium chloride, and chlorhexidine gluconate was still present after contact with these solutions for 30 minutes, while the bacterial growth was completely inhibited by the culture method. Therefore, it was suggested that the bacterial ATP was derived from the coccoid form which was observed morphologically due to the action of these solutions. These results demonstrate that these solutions are not effective bacteriocidally against the coccoid form. Consequently, when we utilize functioning water in the field, we should keep in mind its nonbacteriocidal effect against the coccoid form, as well as its reduced effect under the condition of the contamination by organic substances.
通过培养法（琼脂平板活菌计数）和细菌ATP含量分析（生物发光测定）研究了电解功能水对幽门螺杆菌的杀菌作用。培养法显示该水对马血清和牛血清白蛋白等有机物质的杀菌作用降低。用裂解液处理测定的细菌ATP含量与细菌细胞计数相关。用电解功能水、苯扎氯铵和葡萄糖酸氯己定处理后的细菌，在与这些溶液接触30分钟后仍存在ATP，而通过培养法细菌生长被完全抑制。因此，提示细菌ATP来源于因这些溶液作用在形态学上观察到的球状体形式。这些结果表明这些溶液对球状体形式无有效的杀菌作用。因此，当我们在实际中使用功能水时，应牢记其对球状体形式无杀菌作用，以及在有机物质污染条件下其作用降低的情况。
