Kurokawa M, Nukina M, Nakanishi H, Tomita S, Tamura T, Shimoyama T
Department of Bacteriology, Kobe Institute of Health.
Kansenshogaku Zasshi. 1999 Jan;73(1):15-9. doi: 10.11150/kansenshogakuzasshi1970.73.15.
It is well known that the change of state from the culturable to not culturable on pathogenic organisms could easily occurred. These states of bacteria were so called viable but nonculturable (VNC). The coccoid form of H. pylori is seems to be in this state. The possibility of resuscitation of H. pylori was examined in vitro. The coccoid form bacteria was treated with Ammonium Sulfate and heat shock before culture, and cultured in Brucella broth containing, sodium pyruvate, laked human erythrocyte and serum. The growth of the rod spiral form was found in the coccoid form bacteria, and further the bacteria could grow on blood agar and Skirrow agar as well as the original strain. It was strongly suggested that the resuscitation from VNC state of cells to culturable form consists of two processes, stimulation and supplemented of appropriate nutrition.
众所周知，致病微生物从可培养状态转变为不可培养状态很容易发生。细菌的这些状态被称为活的但不可培养（VNC）状态。幽门螺杆菌的球形形式似乎处于这种状态。在体外研究了幽门螺杆菌复苏的可能性。在培养前，将球形形式的细菌用硫酸铵处理并进行热休克处理，然后在含有丙酮酸钠、裂解的人红细胞和血清的布鲁氏菌肉汤中培养。在球形形式的细菌中发现了杆状螺旋形的生长，并且进一步地，这些细菌能够像原始菌株一样在血琼脂和斯基罗琼脂上生长。强烈提示细胞从VNC状态复苏到可培养形式包括两个过程，即刺激和补充适当的营养。
