Sörberg M, Nilsson M, Hanberger H, Nilsson L E
Department of Infectious Diseases, Danderyd Hospital, Sweden.
Eur J Clin Microbiol Infect Dis. 1996 Mar;15(3):216-9. doi: 10.1007/BF01591357.
The morphologic conversion of Helicobacter pylori from bacillary to coccoid form was studied by microscopy, viable count on agar plates, and bioluminescence assay of bacterial ATP. When morphologic conversion from bacillary to coccoid form was detected by microscopy, the viable counts and the bacterial ATP decreased. No viable count was found after nine days of incubation, but bacterial ATP was still present. In these cultures in which only the coccoid form of Helicobacter pylori was present, there was no accumulation of extracellular ATP, indicating no leaky cells. During the transition phase from the bacillary to the coccoid form of Helicobacter pylori, the addition of fresh medium increased the intracellular ATP 26-fold. The coccoid form of Helicobacter pylori had a 1000-fold lower ATP level per cell compared to the bacillary form, which indicates a decreased metabolic activity in the coccoid form. Addition of fresh medium to the coccoid cultures from days 9 and 10 increased the ATP level twofold. However, no conversion from coccoid to bacillary form was found in these cultures during prolonged incubation in fresh broth for four weeks. Such conversion needs to be demonstrated before it is proven that the coccoid form of Helicobacter pylori is responsible for transmission and relapse of infection.
通过显微镜检查、琼脂平板活菌计数以及细菌ATP的生物发光测定，研究了幽门螺杆菌从杆菌形态向球菌形态的形态学转变。当通过显微镜检测到从杆菌形态向球菌形态的形态学转变时，活菌计数和细菌ATP含量均下降。培养九天后未发现活菌，但细菌ATP仍然存在。在这些仅存在幽门螺杆菌球菌形态的培养物中，没有细胞外ATP的积累，表明没有细胞渗漏。在幽门螺杆菌从杆菌形态向球菌形态的转变阶段，添加新鲜培养基可使细胞内ATP增加26倍。与杆菌形态相比，幽门螺杆菌球菌形态的每个细胞的ATP水平低1000倍，这表明球菌形态的代谢活性降低。在第9天和第10天向球菌培养物中添加新鲜培养基可使ATP水平提高两倍。然而，在新鲜肉汤中长时间培养四周期间，在这些培养物中未发现从球菌形态向杆菌形态的转变。在证明幽门螺杆菌球菌形态与感染的传播和复发有关之前，需要证实这种转变。
