Gonzalez L E, Bashan Y
Department of Biology, Pontificia Universidad Javeriana, Santafe de Bogota, Colombia.
Appl Environ Microbiol. 2000 Apr;66(4):1527-31. doi: 10.1128/AEM.66.4.1527-1531.2000.
Coimmobilization of the freshwater microalga Chlorella vulgaris and the plant-growth-promoting bacterium Azospirillum brasilense in small alginate beads resulted in a significantly increased growth of the microalga. Dry and fresh weight, total number of cells, size of the microalgal clusters (colonies) within the bead, number of microalgal cells per cluster, and the levels of microalgal pigments significantly increased. Light microscopy revealed that both microorganisms colonized the same cavities inside the beads, though the microalgae tended to concentrate in the more aerated periphery while the bacteria colonized the entire bead. The effect of indole-3-acetic acid addition to microalgal culture prior to immobilization of microorganisms in alginate beads partially imitated the effect of A. brasilense. We propose that coimmobilization of microalgae and plant-growth-promoting bacteria is an effective means of increasing microalgal populations within confined environments.
将淡水微藻普通小球藻（Chlorella vulgaris）与促植物生长细菌巴西固氮螺菌（Azospirillum brasilense）共固定在小的藻酸盐珠粒中，可显著促进微藻生长。微藻的干重和鲜重、细胞总数、珠粒内微藻聚集体（菌落）的大小、每个聚集体中的微藻细胞数量以及微藻色素水平均显著增加。光学显微镜观察显示，两种微生物都定殖在珠粒内部的相同腔室中，不过微藻倾向于集中在通气性更好的周边区域，而细菌则定殖在整个珠粒中。在将微生物固定到藻酸盐珠粒之前向微藻培养物中添加吲哚 - 3 - 乙酸，其效果部分类似于巴西固氮螺菌的作用效果。我们认为，微藻与促植物生长细菌的共固定是在受限环境中增加微藻种群数量的有效方法。
