Tanaka A, Nakajima H
Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Japan.
Adv Biochem Eng Biotechnol. 1990;42:97-131. doi: 10.1007/BFb0000732.
Immobilized living and growing cells are attracting worldwide attention because these biocatalysts have self-proliferating and self-regenerating properties of catalytic systems and are able to catalyze efficiently multifunctional and multistep reactions involving coenzyme regeneration. This article summarizes the application of microbial, plant, and mammalian cells, genetically improved or not, immobilized by different methods to the production of amino acids, organic acids, antibiotics, steroids, medicines, enzymes, bioactive peptides, etc., emphasizing the recent results. Effects of the gel properties on the efficient performance of bioprocesses are also discussed.
固定化的活细胞和生长细胞正吸引着全球的关注，因为这些生物催化剂具有催化系统的自我增殖和自我再生特性，并且能够高效催化涉及辅酶再生的多功能和多步反应。本文总结了通过不同方法固定的微生物、植物和哺乳动物细胞（无论是否经过基因改良）在氨基酸、有机酸、抗生素、类固醇、药物、酶、生物活性肽等生产中的应用，重点介绍了近期的研究成果。还讨论了凝胶特性对生物过程高效性能的影响。
