铜形态对 Trichosporium 甲基孢囊菌 OB3b 全细胞可溶性甲烷单加氧酶活性的影响。
Effect of copper speciation on whole-cell soluble methane monooxygenase activity in Methylosinus trichosporium OB3b.
作者信息
Morton J D, Hayes K F, Semrau J D
机构信息
Environmental and Water Resources Engineering, Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan 48109-2125, USA.
出版信息
Appl Environ Microbiol. 2000 Apr;66(4):1730-3. doi: 10.1128/AEM.66.4.1730-1733.2000.
Abstract
Soluble methane monooxygenase (sMMO) activity in Methylosinus trichosporium OB3b was found to be more strongly affected as copper-to-biomass ratios changed in a newly developed medium, M2M, which uses pyrophosphate for metal chelation, than in nitrate mineral salts (NMS), which uses EDTA. When M2M medium was amended with EDTA, sMMO activity was similar to that in NMS medium, indicating that EDTA-bound copper had lower bioavailability than pyrophosphate-bound copper. EDTA did not limit the association of copper with the cells; rather, copper was sequestered in a form which did not affect sMMO activity.
摘要
在新开发的M2M培养基（使用焦磷酸进行金属螯合）中，随着铜与生物量的比例变化，发现 Trichosporium OB3b 甲基单胞菌中的可溶性甲烷单加氧酶（sMMO）活性受到的影响比在使用EDTA的硝酸盐矿物盐（NMS）培养基中更强。当用EDTA对M2M培养基进行改良时，sMMO活性与NMS培养基中的相似，这表明与EDTA结合的铜的生物利用度低于与焦磷酸结合的铜。EDTA并不限制铜与细胞的结合；相反，铜以一种不影响sMMO活性的形式被螯合。
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