使用刚果红染色的琼脂复制品检测聚丙烯酰胺凝胶中的纤维素酶活性。
Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas.
作者信息
Béguin P
出版信息
Anal Biochem. 1983 Jun;131(2):333-6. doi: 10.1016/0003-2697(83)90178-1.
PMID:6193735
Abstract
Bands that have cellulolytic activity are visualized after polyacrylamide gel electrophoresis by laying the slab gel on top of a thin sheet of 2% agar containing 0.1% carboxymethylcellulose. After a suitable incubation time, zones of carboxymethylcellulose hydrolysis are revealed by staining the agar replica with Congo red.
摘要
通过将平板凝胶置于含有0.1%羧甲基纤维素的2%琼脂薄片上，在聚丙烯酰胺凝胶电泳后观察具有纤维素分解活性的条带。经过适当的孵育时间后，用刚果红对琼脂复制品进行染色，以显示羧甲基纤维素水解区域。
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