Henning K, Sachse K, Sting R
Bundesforschungsanstalt für Viruskrankheiten der Tiere, Institut für epidemiologische Diagnostik, Wusterhausen.
Dtsch Tierarztl Wochenschr. 2000 Feb;107(2):49-52.
The isolation and identification of a chlamydial agent from an equine fetus is reported. The fetus was aborted by a mare with respiratory disease and fever in the 9th month of pregnancy. The serum of the mare was investigated by the compliment fixation test. Specific antibodies were detected for chlamydial antigen in a titer of > 1:40 and for equine herpes virus 1 antigen in a titer of 1:32. Pathological lesions were not found in the organs of the fetus. Chlamydiae were detected in the placenta by ELISA and subsequently isolated by cell culture. Using PCR technique the agent was identified as Chlamydophila psittaci.
本文报道了从一匹马胎儿中分离和鉴定出一种衣原体病原体。该胎儿由一匹在怀孕第9个月出现呼吸道疾病和发热症状的母马流产。通过补体结合试验对母马的血清进行了检测。检测到针对衣原体抗原的特异性抗体效价>1:40,针对马疱疹病毒1型抗原的抗体效价为1:32。在胎儿的器官中未发现病理损伤。通过酶联免疫吸附测定法(ELISA)在胎盘中检测到衣原体,随后通过细胞培养将其分离出来。利用聚合酶链反应(PCR)技术,鉴定该病原体为鹦鹉热衣原体。