Asia Pacific Centre for Animal Health, The Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria 3010, Australia.
Department of Medicine (Royal Melbourne Hospital), The University of Melbourne, Parkville, Victoria 3010, Australia.
J Med Microbiol. 2021 Feb;70(2). doi: 10.1099/jmm.0.001284.
is primarily a pathogen of birds but can also cause disease in other species. Equine reproductive loss caused by has recently been identified in Australia where cases of human disease were also reported in individuals exposed to foetal membranes from an ill neonatal foal in New South Wales. The prevalence of in association with equine reproductive over time and in different regions of Australia is not known. This study was conducted to detect in equine abortion cases in Australia using archived samples spanning 25 years. We tested for in 600 equine abortion cases reported in Australia between 1994 to 2019 using a real-time quantitative PCR assay targeting the 16S rRNA gene followed by high-resolution melt curve analysis. Genotyping and phylogenetic analysis was performed on positive samples. The overall prevalence of in material from equine abortion cases was 6.5 %. -positive cases were detected in most years that were represented in this study and occurred in Victoria (prevalence of 7.6 %), New South Wales (prevalence of 3.9 %) and South Australia (prevalence of 15.4 %). Genotyping and phylogenetic analysis showed that the detected in the equine abortion cases clustered with the parrot-associated 6BC clade (genotype A/ST24), indicating that infection of horses may be due to spillover from native Australian parrots. This work suggests that has been a significant agent of equine abortion in Australia for several decades and underscores the importance of taking appropriate protective measures to avoid infection when handling equine aborted material.
是一种主要的鸟类病原体，但也能感染其他物种。最近在澳大利亚发现了由引起的马属动物生殖损失，在新南威尔士州接触患病新生驹胎膜的个体中也报告了人类病例。随着时间的推移和澳大利亚不同地区，与马属动物生殖相关的的流行情况尚不清楚。本研究旨在利用 25 年来的存档样本，检测澳大利亚马流产病例中的。我们使用针对 16S rRNA 基因的实时定量 PCR 检测法，对 1994 年至 2019 年间澳大利亚报告的 600 例马流产病例进行了检测，然后进行高分辨率熔解曲线分析。对阳性样本进行基因分型和系统发育分析。从马流产病例中获得的材料中 的总体流行率为 6.5%。本研究中代表的大多数年份都检测到了 -阳性病例，这些病例发生在维多利亚州（流行率为 7.6%）、新南威尔士州（流行率为 3.9%）和南澳大利亚州（流行率为 15.4%）。基因分型和系统发育分析表明，从马流产病例中检测到的与鹦鹉相关的 6BC 分支（基因型 A/ST24）聚类，表明马的感染可能是由于澳大利亚本土鹦鹉的溢出。这项工作表明，在过去几十年中，已成为澳大利亚马流产的一个重要病原体，并强调了在处理马流产材料时采取适当保护措施避免感染的重要性。
