Wubben J P, ten Have A, van Kan J A, Visser J
Section of Molecular Genetics of Industrial Micro-organisms, Wageningen University, The Netherlands.
Curr Genet. 2000 Feb;37(2):152-7. doi: 10.1007/s002940050022.
The phytopathogenic fungus Botrytis cinerea produces a set of endopolygalacturonases (endoPGs) which are involved in the enzymatic degradation of pectin in plant cell walls. The endoPG-encoding genes of B. cinerea are differentially expressed when the fungus is grown in liquid culture on different carbon sources. A basic constitutive expression level was observed for two genes, Bcpg1 and Bcpg2, which encode basic isozymes. Galacturonic acid was shown to induce the expression of Bcpg4 and Bcpg6. Low pH of the culture medium resulted in induced expression of the Bcpg3 gene. Expression of the Bcpg5 gene was inducible; however the inducing factors could not be identified. Finally, galacturonic acid-induced expression of the Bcpg4 gene was repressed by the presence of more-favourable carbon sources, such as glucose.
植物病原真菌灰葡萄孢产生一组内切多聚半乳糖醛酸酶（endoPGs），这些酶参与植物细胞壁中果胶的酶促降解。当真菌在不同碳源的液体培养基中生长时，灰葡萄孢的endoPG编码基因会有差异地表达。观察到两个编码碱性同工酶的基因Bcpg1和Bcpg2有基本的组成型表达水平。已证明半乳糖醛酸可诱导Bcpg4和Bcpg6的表达。培养基的低pH值导致Bcpg3基因的诱导表达。Bcpg5基因的表达是可诱导的；然而，诱导因子无法确定。最后，更有利的碳源（如葡萄糖）的存在会抑制半乳糖醛酸诱导的Bcpg4基因的表达。
