Hu Y Q, Tan R X, Chu M Y, Zhou J
Department of Biology, Nanjing University, China.
Jpn J Cancer Res. 2000 Jan;91(1):113-7. doi: 10.1111/j.1349-7006.2000.tb00867.x.
The aim of this study was to investigate the effect of water-soluble macromolecular components of Artemisia capillaris Thunberg (ACT) on human hepatoma cell line SMMC-7721 (SMMC-7721). The morphological changes of SMMC-7721 were observed under a light microscope and an electron microscope. Inhibition of proliferation was measured with a colorimetric MTT assay. It was discovered that ACT extract-treated cells exhibit morphological changes typical of apoptosis, including condensed chromatin and a reduction in volume. ACT extract at 25-200 microg/ml dose-dependently inhibited the proliferation of SMMC-7721. The 50% effective dose, evaluated on day 3 of exposure to the extract, was 64.52+/-3.53 microg/ml. Upon gel electrophoresis, the fragmented DNA showed a characteristic ladder pattern. Cell cycle analyses revealed that ACT induced cell cycle arrest at the G0/G1 phase.
本研究旨在探讨茵陈蒿（ACT）的水溶性大分子成分对人肝癌细胞系SMMC-7721（SMMC-7721）的影响。在光学显微镜和电子显微镜下观察SMMC-7721的形态变化。采用比色法MTT测定法检测增殖抑制情况。发现经ACT提取物处理的细胞呈现典型的凋亡形态变化，包括染色质浓缩和体积减小。25-200μg/ml剂量的ACT提取物剂量依赖性地抑制SMMC-7721的增殖。在接触提取物第3天评估的50%有效剂量为64.52±3.53μg/ml。凝胶电泳显示，片段化DNA呈现特征性的梯状条带模式。细胞周期分析表明，ACT诱导细胞周期停滞于G0/G1期。
