Koryntová D, Jelínková L, Rezábek K, Zivný J
Gynek.-porod. klinika 1. LF UK a VFN, Praha.
Ceska Gynekol. 1999 Nov;64(6):383-8.
To review the current status and new trends in assisted reproduction.
The literature on intrauterine insemination, IVF, ICSI, MESA/TESE, preimplantation genetic diagnosis, cryopreservation, in vitro maturation, cloning and stem cells.
Assisted Reproduction Centre, Ist Medical Faculty of Charles University Prague and General Hospital Prague, Apolinárská 18, 128,00, Prague 2, Czech Republic.
IUI, IVF, ICSI also in combination with MESA/TESE are widely used relatively high effective procedures. According to Czech Republic Assisted Reproduction Registry, the pregnancy rate per transfer using IVF, ICSI is 25%, 30% respectively. Cryopreservation of sperm and embryos is a basic part of assisted reproduction, the result with oocytes freezing are still not satisfactory. Preimplantation genetic diagnosis is performed in few countries only due to technical barriers and ethical restrictions. Other methods like cloning, nuclear transfer, embryotherapy or gene manipulation using embryonic stem cells are still only subjects of research.
During the last ten years, assisted reproduction showed rapid development in a number of treated couples and the spectrum of used methods changed radically.
回顾辅助生殖的现状与新趋势。
关于宫内人工授精、体外受精、卵胞浆内单精子注射、经皮附睾精子抽吸术/睾丸精子提取术、植入前遗传学诊断、冷冻保存、体外成熟、克隆及干细胞的文献。
捷克共和国布拉格第二区阿波利纳尔斯卡街18号12800布拉格查尔斯大学第一医学院辅助生殖中心及布拉格综合医院。
宫内人工授精、体外受精、卵胞浆内单精子注射以及与经皮附睾精子抽吸术/睾丸精子提取术联合使用，都是广泛应用且相对高效的方法。根据捷克共和国辅助生殖登记处的数据，体外受精、卵胞浆内单精子注射每次移植的妊娠率分别为25%、30%。精子和胚胎的冷冻保存是辅助生殖的基本组成部分，卵母细胞冷冻的结果仍不尽人意。由于技术障碍和伦理限制，植入前遗传学诊断仅在少数国家开展。其他方法，如克隆、核移植、胚胎治疗或利用胚胎干细胞进行基因操作，仍仅处于研究阶段。
在过去十年中，辅助生殖在接受治疗的夫妇数量方面呈现快速发展，所使用方法的范围也发生了根本性变化。
