Katyal R, Ojha S, Rana S V, Vaiphie K, Singh K, Singh V
Department of Biochemistry, Panjab University, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Ann Nutr Metab. 1999;43(5):319-28. doi: 10.1159/000012800.
Epidemiologic data suggest a close relationship between protein energy malnutrition (or protein energy malnourished) (PEM) and development of infectious diarrheal diseases. In the present studies, the impact of trypsin inhibitor (TI) on the gut following rotavirus (RV) infection was investigated in PEM young growing inbred balb/c mice. The animals were divided into four groups: (1) control; (2) PEM; (3) PEM+RV and (4) PEM+ RV+TI. Control and PEM animals were orally inoculated with normal saline, PEM+RV animals were given 50 microl RV (100ID50) and PEM+RV+TI animals were inoculated with 0.6 mg TI/g body weight + 50 micronl RV. The body weight of each animal was recorded. Six animals from each group were sacrificed on days 0, 1, 3, 5, 7 and 10 post inoculation by cervical dislocation. The intestines were removed, everted and homogenized in ice-cold saline. Leucine aminopeptidase (LAP), glutathione (GSH) levels and L-leucine uptake were determined in the jejunum and ileum. Body weight was decreased in PEM and PEM+RV groups compared to the controls. LAP and leucine uptake levels were elevated in the PEM group but decreased in the PEM+RV group. GSH levels were decreased in the PEM+RV group. Enzyme activity, GSH and LAP uptake levels were restored in the PEM+RV+TI group. The study shows the protective role of soybean TI against RV infection in malnourished mice.
流行病学数据表明，蛋白质能量营养不良（或蛋白质能量营养不足）（PEM）与感染性腹泻疾病的发生之间存在密切关系。在本研究中，在蛋白质能量营养不良的幼年近交系Balb/c小鼠中，研究了胰蛋白酶抑制剂（TI）对轮状病毒（RV）感染后肠道的影响。将动物分为四组：（1）对照组；（2）蛋白质能量营养不良组；（3）蛋白质能量营养不良+轮状病毒组和（4）蛋白质能量营养不良+轮状病毒+胰蛋白酶抑制剂组。对照组和蛋白质能量营养不良组动物口服生理盐水，蛋白质能量营养不良+轮状病毒组动物给予50微升轮状病毒（100个半数感染剂量），蛋白质能量营养不良+轮状病毒+胰蛋白酶抑制剂组动物接种0.6毫克胰蛋白酶抑制剂/克体重+50微升轮状病毒。记录每只动物的体重。接种后第0、1、3、5、7和10天，通过颈椎脱臼处死每组中的6只动物。取出肠道，外翻并在冰冷的盐水中匀浆。测定空肠和回肠中的亮氨酸氨肽酶（LAP）、谷胱甘肽（GSH）水平和L-亮氨酸摄取量。与对照组相比，蛋白质能量营养不良组和蛋白质能量营养不良+轮状病毒组的体重下降。蛋白质能量营养不良组的LAP和亮氨酸摄取水平升高，但蛋白质能量营养不良+轮状病毒组降低。蛋白质能量营养不良+轮状病毒组的GSH水平降低。蛋白质能量营养不良+轮状病毒+胰蛋白酶抑制剂组的酶活性、GSH和LAP摄取水平恢复。该研究表明大豆胰蛋白酶抑制剂对营养不良小鼠的轮状病毒感染具有保护作用。
