Katyal R, Rana S, Singh V, Vaiphei K, Ojha S, Singh K
Department of Gastroenterology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India.
Indian J Exp Biol. 2001 Jun;39(6):546-50.
To investigate the role of soyabean trypsin inhibitor (TI) during rotavirus (RV) diarrhoea, changes in enzyme activities of six relevant mucosal enzymes (lactase, sucrase, maltase, trehalase, glucoamylase and alkaline phosphatase) were assayed following inoculation of suckling mice with EB rotavirus (serotype 3) along with the TI and compared with the age-matched healthy control mice. The animals were divided into three groups i.e. group 1 (controls), group 2 (RV inoculated) and group 3 (RV + TI inoculated and sacrificed under light anaesthesia on 0, 1, 3, 5, 7 and 10 day post inoculation (dpi). Then intestines were excised and divided into two parts (jejunum and ileum). They were separately homogenized in 0.9% cold normal saline and activities of mucosal enzyme were measured. Alkaline phosphatase and disaccharidases were found to be decreased significantly in RV inoculated animals in both the anatomical portions of small intestine of mice. These enzyme levels were restored with the administration of TI i.e. in group 3 and became comparable to the controls in both intestinal portions. These studies suggest that activity of intestinal enzymes which are important in digestive absorptive functions of small intestine were restored with the addition of TI whengiven to infant mice showing its protective efficacy during rotavirus infection.
为了研究大豆胰蛋白酶抑制剂（TI）在轮状病毒（RV）腹泻中的作用，在用EB轮状病毒（3型血清型）联合TI接种乳鼠后，检测了六种相关黏膜酶（乳糖酶、蔗糖酶、麦芽糖酶、海藻糖酶、葡糖淀粉酶和碱性磷酸酶）的酶活性变化，并与年龄匹配的健康对照小鼠进行比较。将动物分为三组，即第1组（对照组）、第2组（接种RV组）和第3组（接种RV + TI组，并在接种后0、1、3、5、7和10天在轻度麻醉下处死）。然后切除肠道并分为两部分（空肠和回肠）。将它们分别在0.9%冷生理盐水中匀浆，并测量黏膜酶的活性。在小鼠小肠的两个解剖部位，接种RV的动物中碱性磷酸酶和双糖酶均显著降低。在第3组中，通过给予TI使这些酶水平恢复，并且在两个肠段中均与对照组相当。这些研究表明，当给感染轮状病毒的幼鼠添加TI时，对小肠消化吸收功能重要的肠道酶活性得以恢复，显示出其在轮状病毒感染期间的保护作用。
