Department of Gastroenterology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Indian J Med Res. 2001 Feb;113:40-7.
the burden of rotavirus disease is significant in developed as well as developing countries and has not diminished with improved hygiene. In the present studies the protective efficacy of soyabean trypsin inhibitor (TI) was investigated on the gut following rotavirus (RV) infection in inbred mice.
infant BALB/c mice of 7 days age (n=108) were divided into three equal groups (control, RV and RV+TI). Animals were orally inoculated either with normal saline, EB RV (serotype-3) or RV+TI and were sacrificed under light chloroform anesthesia on days 0, 1, 3, 5, 7 and 10 post inoculation (pi). Gamma-glutamyl transpeptidase (GGT), leucine aminopeptidase (LAP), and leucine amino acid uptake in the jejunum and ileum were assessed.
rotavirus inoculated animals showed significant reduction in body weights on days 3 and 5 pi as a result of diarrhoea which resolved by day 7 pi. In RV+TI inoculated animals diarrhoea jejunum and ileum in mice inoculated with RV as compared to controls. With the administration of TI the amino acid uptake, and GGT and LAP levels were higher in both segments of the small intestine and comparable to the controls.
on the administration of TI, leucine amino acid uptake, and levels of GGT and LAP were similar to the controls in the jejunum and ileum of infant mice showing its possible protective efficacy during rotavirus infection.
轮状病毒疾病在发达国家和发展中国家的负担都很沉重，而且即使卫生条件得到改善，其负担也没有减轻。在本研究中，我们研究了大豆胰蛋白酶抑制剂（TI）对同源小鼠感染轮状病毒（RV）后的肠道的保护作用。
将 7 天大的婴儿 BALB/c 小鼠（n=108）分为三组（对照组、RV 组和 RV+TI 组）。动物经口接种生理盐水、EB RV（血清型-3）或 RV+TI，并在接种后第 0、1、3、5、7 和 10 天（pi）在轻度氯仿麻醉下处死。评估空肠和回肠中的γ-谷氨酰转肽酶（GGT）、亮氨酸氨肽酶（LAP）和亮氨酸氨基酸摄取。
轮状病毒接种动物在第 3 天和第 5 天出现明显的体重减轻，这是腹泻的结果，腹泻在第 7 天 pi 时得到解决。与对照组相比，RV+TI 接种组的 RV 接种动物的腹泻在空肠和回肠中更为明显。在 TI 给药后，与对照组相比，氨基酸摄取以及 GGT 和 LAP 水平在小肠的两个节段都较高。
在 TI 给药后，婴儿小鼠空肠和回肠中的亮氨酸氨基酸摄取以及 GGT 和 LAP 水平与对照组相似，表明其在轮状病毒感染期间可能具有保护作用。
