Katyal R, Rana S, Vaiphei K, Ojha S, Singh K, Singh V
Department of Gastroenterology, Panjab University, Chandigarh, India.
Ann Nutr Metab. 2000;44(5-6):198-206. doi: 10.1159/000046684.
Rotavirus (RV) is considered to be one of the major causes of acute episodic diarrhoea throughout the world. This study was undertaken to investigate the effect of soybean trypsin inhibitor (TI) on brush-border enzymes during rotavirus infection in protein energy malnourished (PEM) infant mice. Animals were divided into 4 groups, namely controls, PEM, PEM+RV and PEM+RV+TI (n = 36 each). Group 1 and 2 animals were orally inoculated with 50 microl of normal saline each. Group 3 animals were orally inoculated with 50 microl of 100 ID50 dose of RV stock each. Group 4 animals were similarly inoculated with 0.6 mg TI/g body weight along with 50 microl of RV stock each. Animals were examined daily for diarrhoea and their body weight was recorded on alternate days postinoculation (dpi). Animals were killed by cervical dislocation after being given light chloroform anesthesia on 0, 1, 3, 5, 7 and 10 dpi. Small intestines were excised and homogenized in normal saline. Proteins, gammaglutamyl transpeptidase, alkaline phosphatase and disaccharidases were estimated in jejunum and ileum. Body weight was significantly reduced in PEM animals and with RV infection. Histologically, focal areas of vacuolar degeneration of lining epithelium were seen in RV-infected animals. Disaccharidases and other enzyme activities were decreased significantly in the PEM group compared to healthy controls and further depressed with RV infection in malnourished animals as compared to non-infected PEM. The enzyme activities were restored in animals receiving TI along with RV compared to the group receiving RV without TI. With the administration of soybean TI, the activities of disaccharidases, alkaline phosphatase, gammaglutamyl transpeptidase and intestinal architecture were restored showing a protective effect in PEM during RV infection.
轮状病毒（RV）被认为是全球急性发作性腹泻的主要病因之一。本研究旨在调查大豆胰蛋白酶抑制剂（TI）对蛋白质能量营养不良（PEM）幼鼠轮状病毒感染期间刷状缘酶的影响。将动物分为4组，即对照组、PEM组、PEM+RV组和PEM+RV+TI组（每组n = 36）。第1组和第2组动物分别口服接种50微升生理盐水。第3组动物分别口服接种50微升100 ID50剂量的RV毒株。第4组动物同样接种0.6毫克TI/克体重以及50微升RV毒株。每天检查动物是否腹泻，并在接种后隔日（dpi）记录其体重。在接种后0、1、3、5、7和10天，对动物进行轻度氯仿麻醉后通过颈椎脱臼处死。切除小肠并在生理盐水中匀浆。测定空肠和回肠中的蛋白质、γ-谷氨酰转肽酶、碱性磷酸酶和双糖酶。PEM动物以及感染RV后体重显著降低。组织学上，在感染RV的动物中可见衬里上皮细胞空泡变性的局灶区域。与健康对照组相比，PEM组双糖酶和其他酶活性显著降低，与未感染的PEM动物相比，营养不良动物感染RV后酶活性进一步降低。与未接受TI的RV组相比，接受TI和RV的动物酶活性恢复。随着大豆TI的给药，双糖酶、碱性磷酸酶、γ-谷氨酰转肽酶的活性以及肠道结构得以恢复，显示出在RV感染期间对PEM的保护作用。
