Rodríguez J C, Royo G, Rodríguez-Valera F
Sección of Microbiologia, Hospital General Univesitario of Elche, Alicante, Spain.
APMIS. 2000 Mar;108(3):231-6. doi: 10.1034/j.1600-0463.2000.d01-49.x.
We applied four molecular techniques for the typing of strains of Mycobacterium tuberculosis associated with outbreaks: RFLP of the IS6110 insertion sequence, spoligotyping, RAPD, and PCR-IS6110. All 4 techniques were applied to 18 strains which were shown by epidemiological data to be involved in 6 outbreaks. All the methods classified the strains into the same groups as the classical epidemiological data did, but RFLP of the IS6110 insertion sequence and spoligotyping are laborious techniques requiring more than a full day's work, whilst RAPD and PCR IS6110 are simple methods easily incorporated into the daily routine. Nevertheless, a large-scale process of standardization and evaluation is necessary in order to be able to establish the true value of the latter two methods as intraspecific characterization markers for M. tuberculosis isolates.
IS6110插入序列的限制性片段长度多态性分析（RFLP）、间隔寡核苷酸分型（spoligotyping）、随机扩增多态性DNA分析（RAPD）以及PCR-IS6110。这四种技术都应用于18株菌株，根据流行病学数据显示，这些菌株涉及6起疫情。所有方法将菌株分类的结果都与经典流行病学数据一致，但IS6110插入序列的RFLP和间隔寡核苷酸分型是费力的技术，需要一整天多的时间，而RAPD和PCR-IS6110是简单的方法，很容易纳入日常工作流程。然而，为了能够确定后两种方法作为结核分枝杆菌分离株种内特征标记的真正价值，大规模的标准化和评估过程是必要的。
