Plikaytis B B, Kurepina N, Woodley C L, Fleischmann R, Kreiswirth B, Shinnick T M
Division of AIDS, STD and TB Laboratory Research, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Tuber Lung Dis. 1999;79(5):273-8. doi: 10.1054/tuld.1999.0197.
Mycobacterium tuberculosis strain CDC1551 outbreak area in Tennessee and Kentucky and selected locations in the USA.
Develop a PCR assay to distinguish the highly transmissible CDC1551 from strains which have similar 4-band IS6110 fingerprints.
Compare the IS6110 insertion sites in CDC1551 with those in 10 isolates which have similar 4-band IS6110 fingerprints. Utilize unique characteristics of insertion sites in CDC1551 to design a multiplex PCR to identify this strain.
A multiplex PCR was developed which targets an IS6110 insertion conserved in most IS6110 low copy number strains and a deletion within the direct repeat region adjacent to an IS6110 insertion. Of 139 isolates with similar 4-band fingerprints, the CDC1551 PCR pattern was generated by only the 14 outbreak associated isolates. Of 154 isolates with different fingerprints, only four generated the CDC1551 pattern and these could be distinguished from CDC1551 by their IS6110 fingerprint.
The multiplex PCR used in conjunction with the IS6110 fingerprint should be a useful tool to aid in the continued surveillance of the outbreak area and follow the spread of this highly transmissible strain of M. tuberculosis.
田纳西州和肯塔基州的结核分枝杆菌CDC1551菌株爆发地区以及美国的选定地点。
开发一种聚合酶链反应（PCR）检测方法，以区分高传播性的CDC1551菌株与具有相似的4条带IS6110指纹图谱的菌株。
比较CDC1551中IS6110插入位点与10株具有相似4条带IS6110指纹图谱的分离株中的插入位点。利用CDC1551中插入位点的独特特征设计多重PCR以鉴定该菌株。
开发了一种多重PCR，其靶向大多数IS6110低拷贝数菌株中保守的IS6110插入以及与IS6110插入相邻的直接重复区域内的缺失。在139株具有相似4条带指纹图谱的分离株中，仅14株与爆发相关的分离株产生了CDC1551 PCR模式。在154株具有不同指纹图谱的分离株中，只有4株产生了CDC1551模式，并且可以通过它们的IS6110指纹图谱与CDC1551区分开来。
与IS6110指纹图谱结合使用的多重PCR应该是一种有用的工具，有助于对爆发地区进行持续监测，并追踪这种高传播性结核分枝杆菌菌株的传播。
