Ng W L, Ng T P, Kwan H S
Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, PR China.
FEMS Microbiol Lett. 2000 Apr 15;185(2):139-45. doi: 10.1111/j.1574-6968.2000.tb09052.x.
Hydrophobins play important roles in morphogenesis and pathogenesis in fungi and fruit development in mushrooms. Two genes encoding hydrophobins (Le.hyd1 and Le.hyd2) were isolated during sequencing of random clones from a primordial cDNA library of Lentinula edodes. The nucleotide sequences of these two genes were determined. These two genes are 760 and 738 bp in length and the deduced amino acid sequences are homologous to various fungal hydrophobins with characteristic cysteine spacing. These hydrophobin genes are Class I hydrophobins judging by their conserved domains and hydropathy patterns. The transcript level of Le.hyd1 is high in primordium and that of Le.hyd2 is high in dikaryotic mycelial tissues. Poor expression of these two genes in monokaryotic parents indicates that these two genes are under mating-type regulation. We thus suggest that differential expression of these two L. edodes hydrophobins during fruit development may contribute to their distinct roles in fruiting of this mushroom.
疏水蛋白在真菌的形态发生和致病过程以及蘑菇的子实体发育中发挥着重要作用。在对香菇原始cDNA文库的随机克隆进行测序过程中，分离出了两个编码疏水蛋白的基因（Le.hyd1和Le.hyd2）。测定了这两个基因的核苷酸序列。这两个基因的长度分别为760和738 bp，推导的氨基酸序列与具有特征性半胱氨酸间隔的各种真菌疏水蛋白同源。根据其保守结构域和亲水性模式判断，这些疏水蛋白基因属于I类疏水蛋白。Le.hyd1在原基中的转录水平较高，而Le.hyd2在双核菌丝体组织中的转录水平较高。这两个基因在单核亲本中的表达较差，表明这两个基因受交配型调控。因此，我们认为这两种香菇疏水蛋白在子实体发育过程中的差异表达可能有助于它们在这种蘑菇结实过程中发挥不同的作用。
