Takaki Koichi, Yoshida Kohei, Saito Tatsuya, Kusaka Tomohiro, Yamaguchi Ryo, Takahashi Kyusuke, Sakamoto Yuichi
Department of Electrical Engineering, Iwate University, 4-3-5 Ueda, Morioka, Iwate 020-8551, Japan.
Morioka Forest Union, 2-9 Konya, Morioka, Iwate 020-0885, Japan.
Microorganisms. 2014 Feb 12;2(1):58-72. doi: 10.3390/microorganisms2010058.
The effect of high-voltage electrical stimulation on fruit body formation in cultivating mushrooms was evaluated using a compact pulsed power generator designed and based on an inductive energy storage system. An output voltage from 50 to 130 kV with a 100 ns pulse width was used as the electrical stimulation to determine the optimum amplitude. The pulsed high voltage was applied to a sawdust-based substrate of and natural logs hosting , , and . The experimental results showed that the fruit body formation of mushrooms increased 1.3-2.0 times in terms of the total weight. The accumulated yield of for four cultivation seasons was improved from 160 to 320 g by applying voltages of 50 or 100 kV. However, the yield was decreased from 320 to 240 g upon increasing the applied voltage from 100 to 130 kV. The yield of the other types of mushrooms showed tendencies similar to those of when voltage was applied. An optimal voltage was confirmed for efficient fruit body induction. The hypha activity was evaluated by the amount of hydrophobin release, which was mainly observed before the fruit body formation. The hydrophobin release decreased for three hours after stimulation. However, the hydrophobin release from the vegetative hyphae increased 2.3 times one day after the stimulation.
使用基于感应储能系统设计的紧凑型脉冲电源发生器，评估了高压电刺激对栽培蘑菇子实体形成的影响。输出电压为50至130 kV，脉冲宽度为100 ns，用作电刺激以确定最佳幅度。将脉冲高压施加到含有香菇、平菇、金针菇和杏鲍菇的木屑基基质和天然原木上。实验结果表明，蘑菇子实体形成的总重量增加了1.3至2.0倍。通过施加50或100 kV的电压，四个栽培季节的香菇累计产量从160克提高到320克。然而，当施加电压从100 kV增加到130 kV时，产量从320克下降到240克。施加电压时，其他类型蘑菇的产量表现出与香菇相似的趋势。确定了有效诱导子实体的最佳电压。通过疏水蛋白释放量评估菌丝活性，疏水蛋白释放主要在子实体形成之前观察到。刺激后三小时内疏水蛋白释放减少。然而，刺激一天后，营养菌丝的疏水蛋白释放增加了2.3倍。
