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Structural heterogeneity in the O polysaccharide of Pseudomonas syringae pv. coriandricola GSPB 2028 (NCPPB 3780, W-43).

作者信息

Knirel Y A, Ovod V V, Paramonov N A, Krohn K J

机构信息

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.

出版信息

Eur J Biochem. 1998 Dec 1;258(2):716-21. doi: 10.1046/j.1432-1327.1998.2580716.x.

DOI:10.1046/j.1432-1327.1998.2580716.x

PMID:9874239

Abstract

The O polysaccharide (OPS) of the lipopolysaccharide of Pseudomonas syringae pv. coriandricola GSPB 2028 (NCPPB 3780, W-43) was studied by Smith degradation and 1H-NMR and 13C-NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, and H-detected 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments. The OPS was shown to consist of pentasaccharide O repeats of two types both containing four L-rhamnose and one 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc) residue. Structure 1 of the major O repeat which had been established earlier [Das, S., Ramm, M., Kochanowski, H. & Basu, S. (1994) J. Bacteriol. 176, 6550-6557], was confirmed by our data, and a new structure 2 was elucidated for the minor O repeat and found to differ from the structure 1 only in the position of substitution of one of the rhamnose residues in the main chain. [structures: see text] A role of structural and immunochemical features of the LPS for defining the taxonomical position of the bacterium studied is discussed.

摘要

通过史密斯降解法以及一维氢核磁共振（¹H-NMR）和碳-13核磁共振（¹³C-NMR）光谱法，包括二维化学位移相关谱（COSY）、全相关谱（TOCSY）、核Overhauser效应谱（NOESY）以及氢检测的氢-碳异核多量子相干（HMQC）实验，对丁香假单胞菌芫荽致病变种GSPB 2028（NCPPB 3780，W-43）脂多糖的O抗原多糖（OPS）进行了研究。结果表明，OPS由两种类型的五糖O重复单元组成，每个重复单元均包含四个L-鼠李糖和一个3-乙酰氨基-3,6-二脱氧-D-半乳糖（D-Fuc3NAc）残基。我们的数据证实了先前确定的主要O重复单元的结构1[达斯，S.，拉姆，M.，科哈诺夫斯基，H. & 巴苏，S.（1994年）《细菌学杂志》176，6550 - 6557]，并阐明了次要O重复单元的新结构2，发现其与结构1的区别仅在于主链中一个鼠李糖残基的取代位置。[结构：见正文] 讨论了脂多糖的结构和免疫化学特征在确定所研究细菌分类地位方面的作用。