Haughton G, Daly J A, Wikstrand C J
Transplantation. 1979 Mar;27(3):203-7.
The extremely sensitive peritoneal exudate cell (PEC) transfer technique has been applied to an investigation of the immune response of female mice which are "tolerant" of the male antigen. Females rendered tolerant of male skin grafts by multiparity, neonatal inoculation of male spleen cells, or multiple inoculations of adult females with male lymphoid cells displayed second-set reactivity to male PEC while continuing to tolerate H-Y-incompatible skin grafts. Furthermore, this discordant response to male PEC and skin was adoptively transferrable to normal females by spleen cells from multiparous donors. Females rendered tolerant by irradiation and reconstitution with male cells were unresponsive to both H-Y-incompatible skin and PEC grafts. A model is proposed using two male-specific antigens, the response to which is controlled by independent genes.
极其敏感的腹膜渗出细胞（PEC）转移技术已被应用于对雌性小鼠免疫反应的研究，这些雌性小鼠对雄性抗原“耐受”。通过多胎妊娠、新生期接种雄性脾细胞或成年雌性多次接种雄性淋巴细胞而对雄性皮肤移植产生耐受的雌性小鼠，在继续耐受H-Y不相容皮肤移植的同时，对雄性PEC表现出二次反应性。此外，这种对雄性PEC和皮肤的不一致反应可通过多胎供体的脾细胞过继转移给正常雌性小鼠。经照射并用雄性细胞重建而产生耐受的雌性小鼠，对H-Y不相容皮肤和PEC移植均无反应。提出了一个使用两种雄性特异性抗原的模型，对这两种抗原的反应由独立基因控制。
