Clavijo A, Munroe F, Zhou E M, Booth T F, Roblesky K
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Manitoba.
Can Vet J. 2000 Apr;41(4):312-4.
Bluetongue virus was isolated from a sentinel herd in British Columbia. Virus isolation was by intravenous inoculation of embryonated chicken eggs and subculture in BHK-21 cells. The cytopathic agent was identified as bluetongue virus by electron microscopy and the immunoperoxidase test. The serotype was identified as serotype 11 by virus neutralization.
蓝舌病毒是从不列颠哥伦比亚省的一个哨兵畜群中分离出来的。病毒分离是通过将其静脉接种到鸡胚中,并在BHK - 21细胞中进行传代培养。通过电子显微镜和免疫过氧化物酶试验,将细胞病变因子鉴定为蓝舌病毒。通过病毒中和试验,将血清型鉴定为11型。