Mislovicová D, Masárová J, Bendzálová K, Soltés L, Machová E
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Ultrason Sonochem. 2000 Apr;7(2):63-8. doi: 10.1016/s1350-4177(99)00030-9.
A water-insoluble chitin-glucan complex, isolated from the mycelium of Aspergillus niger, was swollen in various aqueous media and treated subsequently by high-energy sonication. The concentration of the resulting water-soluble polysaccharide fractions was dependent on the swelling medium, the amount of the chitin-glucan complex in the suspension, and on the time of sonication. The yields of water-soluble chitin-glucan were within the range 13.6 to 24.4% relative to the mass of the original chitin-glucan. The nitrogen content obtained for the samples of water-soluble chitin-glucan indicated a higher content of chitin (3.45% of nitrogen in high-molecular fraction) than in the original water-insoluble chitin-glucan sample (1.8%). The distribution of the molecular weights of the water-soluble fractions prepared was determined by high-performance liquid chromatography.
从黑曲霉菌丝体中分离出的一种水不溶性几丁质-葡聚糖复合物，在各种水性介质中溶胀，随后进行高能超声处理。所得水溶性多糖级分的浓度取决于溶胀介质、悬浮液中几丁质-葡聚糖复合物的量以及超声处理时间。相对于原始几丁质-葡聚糖的质量，水溶性几丁质-葡聚糖的产率在13.6%至24.4%的范围内。水溶性几丁质-葡聚糖样品的氮含量表明，其几丁质含量（高分子级分中氮含量为3.45%）高于原始水不溶性几丁质-葡聚糖样品（1.8%）。通过高效液相色谱法测定所制备的水溶性级分的分子量分布。
