Artamonova S D, Sharnina F F
Prikl Biokhim Mikrobiol. 2013 Jul-Aug;49(4):417-22. doi: 10.7868/s0555109913030045.
The peculiarities of the acidic hydrolysis of chitin-glucan complexes (CGCs) of higher fungi were studied, and the technology for the isolation and purification of D-(+)-glucosamine hydrochloride of high purity from hydrolysate was developed. The composition, properties, and purity of the product were analyzed by a combination of physicochemical methods. The yield of the final product was 20-60%, depending on the chitin content in CGC samples. The amino sugar obtained was a white crystalline odorless powder readily soluble in water, slightly soluble in 95% ethanol, and insoluble in chloroform and other organic solvents. It corresponds to the standard D-(+)-glucosamine hydrochloride in the main qualitative indicators.
研究了高等真菌几丁质 - 葡聚糖复合物（CGCs）酸性水解的特性，并开发了从水解产物中分离和纯化高纯度D-(+)-氨基葡萄糖盐酸盐的技术。通过多种物理化学方法相结合对产物的组成、性质和纯度进行了分析。最终产物的产率为20 - 60%，这取决于CGC样品中的几丁质含量。所获得的氨基糖为白色结晶性无臭粉末，易溶于水，微溶于95%乙醇，不溶于氯仿和其他有机溶剂。在主要定性指标方面，它与标准D-(+)-氨基葡萄糖盐酸盐相符。
