Machová E, Kvapilová K, Kogan G, Sandula J
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38 Bratislava, Slovak Republic.
Ultrason Sonochem. 1999 Jan;5(4):169-72. doi: 10.1016/s1350-4177(98)00045-5.
Different factors affecting the efficiency of the ultrasonic depolymerization of the high-molecular-weight carboxymethylated chitin-glucan prepared from the fungal mycelium of Aspergillus niger have been investigated. The influence of the following parameters was examined: concentration of the chitin-glucan complex, duration of ultrasonic irradiation, reaction temperature and volume of the ultrasonicated solution. The optimized conditions for the efficient ultrasonic depolymerization include: polysaccharide concentration--0.2 mg ml-1; volume of the sonicated solution--25 ml; duration of the sonication--10 min; and constant cooling of the sonicated sample in an ice-water bath.
研究了影响从黑曲霉真菌菌丝体制备的高分子量羧甲基化几丁质-葡聚糖超声解聚效率的不同因素。考察了以下参数的影响：几丁质-葡聚糖复合物的浓度、超声辐照时间、反应温度和超声处理溶液的体积。高效超声解聚的优化条件包括：多糖浓度——0.2 mg/ml；超声处理溶液的体积——25 ml；超声处理时间——10分钟；以及在冰水浴中对超声处理后的样品持续冷却。
