Matsubara H, Michitaka K, Horiike N, Yano M, Akbar S M, Torisu M, Onji M
Third Department of Internal Medicine, Ehime University School of Medicine, Shigenobu-cho, Ehime, Japan.
Intervirology. 2000;43(1):16-9. doi: 10.1159/000025018.
Although recent studies indicate a high prevalence (12-92%) of TT virus (TTV) DNA in sera of healthy Japanese individuals, there is a paucity of information regarding the route of transmission of this virus. Analyzing the nucleotide sequences of the existing polymerase chain reaction (PCR) primers of TTV DNA, we developed a set of noble primers (HM-1) and looked for the prevalence of TTV DNA in sera from 39 normal healthy Japanese individuals using PCR. The existence of TTV DNA was also checked in saliva, urine, sweat, stool, and tears from 11 and in semen from 10 serum TTV-positive normal subjects. TTV DNA was detected in sera from 23 of 39 (59.0%) normal subjects. TTV DNA was also detected in saliva, stool, semen and tears from all cases with TTV-DNA-positive serum, but not in body fluids from subjects with TTV-DNA-negative serum. TTV DNA remained undetected in urine and sweat from all cases. Data from these experiments showing the existence of TTV DNA in different body fluids suggest that the high rates of prevalence of TTV among normal healthy subjects might be due to a possible fecal-oral, droplet, or sexual route of transmission of TTV.
尽管最近的研究表明，健康日本个体血清中TT病毒（TTV）DNA的流行率很高（12%-92%），但关于该病毒的传播途径的信息却很少。通过分析现有的TTV DNA聚合酶链反应（PCR）引物的核苷酸序列，我们开发了一组新型引物（HM-1），并使用PCR检测了39名正常健康日本个体血清中TTV DNA的流行情况。我们还检测了11名血清TTV阳性正常受试者的唾液、尿液、汗液、粪便、眼泪以及10名血清TTV阳性正常受试者的精液中的TTV DNA。在39名正常受试者中，有23名（59.0%）的血清检测到TTV DNA。所有血清TTV-DNA阳性病例的唾液、粪便、精液和眼泪中也检测到了TTV DNA，但血清TTV-DNA阴性受试者的体液中未检测到。所有病例的尿液和汗液中均未检测到TTV DNA。这些实验数据表明不同体液中存在TTV DNA，这提示正常健康受试者中TTV的高流行率可能是由于TTV可能通过粪-口、飞沫或性传播途径传播。
