Paraskevis D, Magiorkinis E, Theodoridou M, Mostrou G, Papaevangelou V, Kiosses V G, Hatzakis A, Matsaniotis N
Department of Hygiene and Epidemiology, Athens University Medical School, Greece.
J Hum Virol. 1999 Nov-Dec;2(6):339-43.
To investigate the subtype classification of the circulating virus strains among human immunodeficiency virus type 1 (HIV-1)-infected children in Greece.
STUDY DESIGN/METHODS: Since the beginning of the acquired immunodeficiency syndrome (AIDS) epidemic in Greece in 1982, 23 children have been reported to be vertically infected with HIV-1. Blood samples were available for 19 of these children, and the C2-C4 env region was successfully amplified by nested polymerase chain reaction (PCR) for 16 subjects. HIV-1 subtype was established by the heteroduplex mobility assay (HMA) in 16 subjects and confirmed by DNA sequencing and phylogenetic analysis in 8 subjects.
Most subjects (9; 56%) fell into subtype B. However, a substantial proportion (44%) were classified as subtypes A (3; 19%), C (1; 6%), D (1; 6%), and I (2; 12%). According to epidemiologic information, 5 of 7 children infected with non-B HIV-1 subtypes were born to Greek parents.
These findings clearly suggest that non-B strains have been introduced into Greece, providing evidence that HIV epidemic in this country will probably change profile over time. In addition, subtype I was identified in 2 HIV-1-infected children, both of whom were born to Greek parents.
调查希腊人类免疫缺陷病毒1型（HIV-1）感染儿童中循环病毒株的亚型分类。
研究设计/方法：自1982年希腊艾滋病流行开始以来，已有23名儿童被报告为垂直感染HIV-1。其中19名儿童有血样可用，16名受试者通过巢式聚合酶链反应（PCR）成功扩增了C2-C4 env区域。16名受试者通过异源双链迁移率分析（HMA）确定了HIV-1亚型，8名受试者通过DNA测序和系统发育分析进行了确认。
大多数受试者（9名；56%）属于B亚型。然而，相当一部分（44%）被分类为A（3名；19%）、C（1名；6%）、D（1名；6%）和I（2名；12%）亚型。根据流行病学信息，7名感染非B型HIV-1亚型的儿童中有5名的父母是希腊人。
这些发现清楚地表明非B型毒株已传入希腊，这证明该国的HIV流行情况可能会随时间而改变。此外，在2名HIV-1感染儿童中鉴定出I亚型，这两名儿童的父母均为希腊人。
