Sanchez Gloria I, Bautista Christian T, Eyzaguirre Lindsay, Carrion Gladys, Arias Sonia, Sateren Warren B, Negrete Monica, Montano Silvia M, Sanchez Jose L, Carr Jean K
Grupo Infección y Cáncer, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
Am J Trop Med Hyg. 2006 Apr;74(4):674-7.
To study the molecular epidemiology of human immunodeficiency virus type 1 (HIV-1) strains in Medellín, Colombia, 115 HIV-1-positive individuals who were recruited from an HIV outpatient hospital (Universitario San Vicente de Paul) during the period from July 2001 to January 2002 were genotyped. All samples were analyzed by envelope heteroduplex mobility assay and found to be subtype B. Twenty-four samples were randomly selected for sequencing of the protease and the reverse transcriptase regions; all isolates were found to be subtype B. Phylogenetic analysis of seven nearly full-length genomes showed that all samples were subtype B. This study shows that the HIV epidemic in Colombia continues to be dominated by the subtype B virus. The predominance of subtype B genotypes of HIV-1 strains in Medellín resembles what is seen in the nearby countries of Peru, Ecuador, and Venezuela.
为研究哥伦比亚麦德林市1型人类免疫缺陷病毒（HIV-1）毒株的分子流行病学，对2001年7月至2002年1月期间从一家HIV门诊医院（圣维森特·德·保罗大学医院）招募的115名HIV-1阳性个体进行了基因分型。所有样本均通过包膜异源双链迁移率分析进行检测，结果均为B亚型。随机选取24个样本对蛋白酶和逆转录酶区域进行测序；所有分离株均为B亚型。对七个近乎全长的基因组进行系统发育分析表明，所有样本均为B亚型。本研究表明，哥伦比亚的HIV疫情仍然以B亚型病毒为主导。麦德林市HIV-1毒株B亚型基因型的优势情况与附近国家秘鲁、厄瓜多尔和委内瑞拉相似。
