Kochkina Z M, Chirkov S N
Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
Mikrobiologiia. 2000 Mar-Apr;69(2):266-9.
The influence of chitosan fragments with different degrees of polymerization and some chemical chitosan derivatives on the infection of Bacillus thuringiensis by phage 1-97A was studied. It was shown that chitosan inhibits phage infection and inactivates phage particles. The extent of inhibition of phage infection inversely depended on the degree of polymerization of chitosan fragments. On the contrary, the extent of inactivation of phage virulence was proportional to the degree of polymerization. Chitosan derivatives did not inhibit the growth of bacilli. Deaminated chitosan derivatives at a concentration of 100 mg/ml efficiently inhibited phage reproduction, exhibiting no correlation between the degree of deamination and antiviral activity. The anionic derivative chitosan sulfate and N-succinate-6-O-sulfate did not inactivate phage, did not influence bacterial growth, and did not inhibit the process of viral infection.
研究了不同聚合度的壳聚糖片段以及一些化学壳聚糖衍生物对噬菌体1-97A感染苏云金芽孢杆菌的影响。结果表明，壳聚糖可抑制噬菌体感染并使噬菌体颗粒失活。噬菌体感染的抑制程度与壳聚糖片段的聚合度呈反比。相反，噬菌体毒力的失活程度与聚合度成正比。壳聚糖衍生物不抑制芽孢杆菌的生长。浓度为100mg/ml的脱氨基壳聚糖衍生物能有效抑制噬菌体繁殖，脱氨基程度与抗病毒活性之间无相关性。阴离子衍生物硫酸壳聚糖和N-琥珀酸-6-O-硫酸盐不会使噬菌体失活，不影响细菌生长，也不抑制病毒感染过程。
