Muraguchi H, Kamada T
Department of Biology, Faculty of Science, Okayama, 700-8530, Japan.
Fungal Genet Biol. 2000 Feb;29(1):49-59. doi: 10.1006/fgbi.2000.1184.
A dominant mutation of the elongationless2 (eln2) gene of the mushroom Coprinus cinereus (eln2-1) affects pattern formation in the development of fruit body primordia, causing dumpy primordia which culminate in mature fruit bodies with short stipes. Phenotypic analyses revealed disturbance of tissue organization and cell morphogenesis in the primordial shaft and suggested that the defects in the primordial shaft interfere with differentiation of the stipe tissue in the mutant primordia. The eln2 locus was assigned to chromosome XII and the eln2-1 mutant gene was cloned from a chromosome XII-specific cosmid library as a genomic fragment that brings about the dominant mutant phenotype. The eln2 gene encodes a novel type of microsomal cytochrome P450 enzyme (CYP502) and is constitutively expressed. The eln2-1 mutation is a 4-bp deletion in the 3'-terminal region of the gene, leading to truncation of C-terminal 18 amino acids. We suggest that the truncation affects the activity of the CYP502 enzyme.
灰盖鬼伞（Coprinus cinereus）的无伸长2（eln2）基因的显性突变（eln2-1）影响子实体原基发育中的模式形成，导致矮小的原基，最终发育成菌柄短的成熟子实体。表型分析揭示了原基轴中组织组织和细胞形态发生的紊乱，并表明原基轴中的缺陷干扰了突变原基中菌柄组织的分化。eln2基因座被定位到第十二号染色体，并且从第十二号染色体特异性黏粒文库中克隆出eln2-1突变基因，作为一个导致显性突变表型的基因组片段。eln2基因编码一种新型的微粒体细胞色素P450酶（CYP502）并且持续表达。eln2-1突变是该基因3'末端区域的4个碱基缺失，导致C末端18个氨基酸的截短。我们认为这种截短影响了CYP502酶的活性。
