Wang Peng, Yu Ya, Xia Lei, Yan Qi, Tan Xiao, Wang Dongyin, Wang Xue, Zhang Zhibin, Wen Jiawei, Huang Xiao
Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, China.
College of Horticulture, Jilin Agricultural University, Changchun 130118, China.
J Fungi (Basel). 2025 Jul 8;11(7):512. doi: 10.3390/jof11070512.
Fruiting body formation in edible fungi is a critical development process for both scientific understanding and industrial cultivation, yet the underlying genetic mechanisms remain poorly elucidated. This study aimed to identify key genes regulating monokaryotic fruiting in . A genetic segregation population was constructed through selfing purification and hybrid segregation of the FF002 strain, followed by mapping candidate genes with bulked segregant analysis sequencing (BSA-seq). A 10 kb genomic region on scaffold19 was identified, pinpointing the gene , which encodes a U2 snRNP complex component involved in pre-mRNA splicing. A T→G SNP located 121 bp downstream of the ATG codon caused a serine-to-alanine substitution, disrupting a conserved domain and altering fruiting phenotypes. Phylogenetic analysis further revealed conservation of this gene in fungal genera. These findings elucidate a key regulatory gene controlling monokaryotic fruiting in , providing novel insights into fruiting body formation mechanisms and establishing a foundation for genetic studies in other edible fungi.
食用菌子实体的形成对于科学理解和工业栽培而言都是一个关键的发育过程，但其潜在的遗传机制仍未得到充分阐明。本研究旨在鉴定调控[具体食用菌名称未给出]单核体结实的关键基因。通过FF002菌株的自交纯化和杂交分离构建了一个遗传分离群体，随后利用混合分组分析法测序（BSA-seq）对候选基因进行定位。在scaffold19上鉴定出一个10 kb的基因组区域，确定了基因[具体基因名称未给出]，该基因编码参与前体mRNA剪接的U2 snRNP复合体成分。位于ATG密码子下游121 bp处的一个T→G单核苷酸多态性导致丝氨酸到丙氨酸的替换，破坏了一个保守结构域并改变了结实表型。系统发育分析进一步揭示了该基因在真菌属中的保守性。这些发现阐明了一个控制[具体食用菌名称未给出]单核体结实的关键调控基因，为子实体形成机制提供了新的见解，并为其他食用菌的遗传研究奠定了基础。
