Kamali-Moghaddam M, Sundström L
Department of Pharmaceutical Biosciences, Division of Microbiology, Uppsala University, Biomedicum, SE-751 23, Uppsala, Sweden.
FEMS Microbiol Lett. 2000 May 1;186(1):55-9. doi: 10.1111/j.1574-6968.2000.tb09081.x.
The Mu-related transposon Tn5090, also called Tn402, was observed to be highly selective for targets clustered in or close to recombination sites of serine-type recombinases in plasmids R388 and RP1. Transposition to the par area of RP1 responded strongly to a deletion in the gene of resolvase ParA. A search in sequence databanks revealed further insertions of Tn5090/Tn402 close to different genes of resolvases. These results imply that the target selection of Tn5090 depends on a property that is shared among several serine recombinases.
与Mu相关的转座子Tn5090，也称为Tn402，被观察到对聚集在质粒R388和RP1中丝氨酸型重组酶的重组位点内或附近的靶标具有高度选择性。向RP1的par区域转座对解离酶ParA基因中的缺失有强烈反应。在序列数据库中搜索发现Tn5090/Tn402在解离酶的不同基因附近有进一步插入。这些结果表明Tn5090的靶标选择取决于几种丝氨酸重组酶共有的一种特性。
