Singh I, Carillo O, Namboodiri A
Department of Pediatrics, Medical University of South Carolina, Charleston 29425, USA.
Neurochem Res. 2000 Feb;25(2):197-203. doi: 10.1023/a:1007563201595.
Peroxisomes are now recognized to play important cellular functions and its dysfunction leads to a group of neurological disorders. This study reports peroxisomal enzyme activities in cultured glial cells and peroxisomes isolated from cultured oligodendrocytes and C6 glial cells. Peroxisomal enzyme activities were found to be higher in oligodendroglial cells than in astrocytes or mixed glial cells. We also developed a method for the isolation of peroxisomes from glial cells by a combination of differential and density gradient centrifugation techniques. Peroxisomes from oligodendrocytes in nycodenz gradient were isolated at a density of 1.165 g/ml +/- 0.011. Activities of dihydroxyacetone phosphate acyl transferase, beta-oxidation of lignoceric acid and alpha-oxidation of phytanic acid were almost exclusively associated with the distribution of catalase activity (a marker enzyme for peroxisomes) in the gradient. This protocol should be a resource for studies designed to investigate the structure and function of peroxisomes in brain cells.
过氧化物酶体目前被认为具有重要的细胞功能，其功能障碍会导致一系列神经疾病。本研究报告了培养的神经胶质细胞中的过氧化物酶体酶活性，以及从培养的少突胶质细胞和C6神经胶质细胞中分离出的过氧化物酶体的酶活性。研究发现，少突胶质细胞中的过氧化物酶体酶活性高于星形胶质细胞或混合神经胶质细胞。我们还开发了一种通过差速离心和密度梯度离心技术相结合的方法，从神经胶质细胞中分离过氧化物酶体。在尼可登密度梯度中，少突胶质细胞的过氧化物酶体以1.165 g/ml±0.011的密度被分离出来。磷酸二羟丙酮酰基转移酶活性、木蜡酸的β-氧化和植烷酸的α-氧化几乎完全与梯度中过氧化氢酶活性（过氧化物酶体的标记酶）的分布相关。该方案应为旨在研究脑细胞中过氧化物酶体结构和功能的研究提供资源。
