定量聚合酶链反应和分支DNA检测法用于检测肝细胞癌患者及肝移植患者血清中乙肝病毒DNA的评估
Evaluation of quantitative PCR and branched-chain DNA assay for detection of hepatitis B virus DNA in sera from hepatocellular carcinoma and liver transplant patients.
作者信息
Chen T, Luk J M, Cheung S T, Yu W C, Fan S T
机构信息
Center of Liver Diseases and Department of Surgery, University of Hong Kong Medical Center, Queen Mary Hospital, Pokfulam, Hong Kong.
出版信息
J Clin Microbiol. 2000 May;38(5):1977-80. doi: 10.1128/JCM.38.5.1977-1980.2000.
Abstract
This study evaluated the applicability of quantitative PCR (Q-PCR) and branched-chain DNA assays for detection of hepatitis B virus (HBV) DNA in sera. For 42 samples, the detection rates were 81 and 41%, respectively, with a correlation coefficient of 0.633. The Q-PCR is useful for early monitoring of HBV load in high-risk patients.
摘要
本研究评估了定量聚合酶链反应（Q-PCR）和分支链DNA检测法在血清中检测乙型肝炎病毒（HBV）DNA的适用性。对于42份样本，检测率分别为81%和41%，相关系数为0.633。Q-PCR有助于对高危患者的HBV载量进行早期监测。
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