吸烟和不吸烟正常受试者血清、肺泡巨噬细胞及肺泡灌洗液中的α1-抗胰蛋白酶含量。
Alpha-1-antitrypsin content in the serum, alveolar macrophages, and alveolar lavage fluid of smoking and nonsmoking normal subjects.
作者信息
Olsen G N, Harris J O, Castle J R, Waldman R H, Karmgard H J
出版信息
J Clin Invest. 1975 Feb;55(2):427-30. doi: 10.1172/JCI107947.
Abstract
The content of alpha-1-antitrypsin in the serum, alveolar lavage fluid, and alveolar macrophages of smokers and nonsmokers was studied. Bronchoalveolar lavage was used to obtain alveolar fluid and macrophages from normal volunteers, and alpha-1-antitrypsin and albumin were measured using the electroimmunodiffusion technique. The serum level of inhibitor was not different between the two groups, while the total lavage fliud content of alpha-1-antitrypsin was increased in the smokers. The level of alpha-1-antitrypsin was also significantly greater (P less than 0.001) in the alveolar macrophages of the smokers suggesting the possibility of chronically increased alveolar levels in the cigarette smoker as a possible protective mechanism against proteolysis.
摘要
对吸烟者和非吸烟者血清、肺泡灌洗液及肺泡巨噬细胞中的α-1-抗胰蛋白酶含量进行了研究。采用支气管肺泡灌洗术从正常志愿者获取肺泡液和巨噬细胞，并用免疫电扩散技术测定α-1-抗胰蛋白酶和白蛋白。两组间抑制剂的血清水平无差异，而吸烟者肺泡灌洗液中α-1-抗胰蛋白酶的总含量增加。吸烟者肺泡巨噬细胞中α-1-抗胰蛋白酶水平也显著更高（P<0.001），提示吸烟者肺泡中该物质水平长期升高可能是一种针对蛋白水解的保护机制。
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