Fujita J, Bungo M, Hata Y, Nakamura H, Shiotani T, Irino S
Nihon Kyobu Shikkan Gakkai Zasshi. 1989 Jun;27(6):712-7.
This study was designed to evaluate the elastase: alpha 1-antitrypsin balance in patients with bacterial pneumonia. To accomplish this, bronchoalveolar lavages were performed in patients with pneumonia and normal non-smokers. Cell differentiation counts and elastase/alpha 1-antitrypsin balances in alveolar lavage fluid were examined. To quantify antielastase capacity, total immunoreactive alpha 1-antitrypsins and active alpha 1-antitrypsins were measured in alveolar lavage fluids. To quantify elastase burden, elastase: alpha 1-antitrypsin complexes and enzymatic elastase activities in alveolar lavage fluids were measured. Significantly increased proportions of neutrophils were recovered from the alveolar lavage fluids of patients with pneumonia compared to normal non-smokers. In alveolar lavage fluids of patients with pneumonia, alpha 1-antitrypsin was inactivated and enzymatic elastase activities were demonstrated. It was concluded that elastase: alpha 1-antitrypsin imbalance do occur in alveolar lavage fluids of patients with pneumonia.
本研究旨在评估细菌性肺炎患者体内弹性蛋白酶与α1抗胰蛋白酶的平衡状态。为此，对肺炎患者及正常不吸烟者进行了支气管肺泡灌洗。检测了肺泡灌洗液中的细胞分化计数以及弹性蛋白酶/α1抗胰蛋白酶平衡。为量化抗弹性蛋白酶能力，测定了肺泡灌洗液中总免疫反应性α1抗胰蛋白酶和活性α1抗胰蛋白酶。为量化弹性蛋白酶负荷，测定了肺泡灌洗液中弹性蛋白酶-α1抗胰蛋白酶复合物及酶促弹性蛋白酶活性。与正常不吸烟者相比，肺炎患者肺泡灌洗液中回收的中性粒细胞比例显著增加。在肺炎患者的肺泡灌洗液中，α1抗胰蛋白酶失活且显示出酶促弹性蛋白酶活性。得出的结论是，肺炎患者的肺泡灌洗液中确实存在弹性蛋白酶-α1抗胰蛋白酶失衡。
