Funakoshi N, Onizuka M, Yanagi K, Ohshima N, Tomoyasu M, Sato Y, Yamamoto T, Ishikawa S, Mitsui T
Department of Surgery, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan.
Microvasc Res. 2000 May;59(3):361-7. doi: 10.1006/mvre.2000.2238.
We created anexperimental model of pulmonary metastasis based on subcutaneously implanted Lewis lung cancer in mice and observed in vivo the microcirculation of spontaneously metastasized tumors in the lung. The mice lung was held by a small handmade suction ring to stop cardiac and respiratory movement. Using fluorescent microscopy, tumor microcirculation and normal lung microcirculation in the same lung lobe were compared by measuring microvessel diameter and blood flow velocity [red blood cell (rbc) velocity]. In normal microcirculation, the mean values of microvessel diameter and rbc velocity were 10.4 +/- 2.7 microm and 188 +/- 63 microm/s, respectively. In tumor microcirculation, the mean values of the same were 10.6 +/- 3.3 microm and 105 +/- 40 microm/s. The rbc velocity in normal microcirculation was significantly higher (P < 0.001) than that in tumor microcirculation. The calculated shear rates of normal microcirculation and tumor microcirculation were 73.4 +/- 23.4 (/s) and 41.2 +/- 16.1 (/s), respectively. The shear rate of the tumor microcirculation was significantly slower (P < 0.001) than that of the normal microcirculation. We demonstrated a feasibility of observation and measurement of tumor microcirculation in the lung and confirmed that the physiologic data were compatible to those in the brain or in the liver reported by others. This model might be useful for studying metastatic tumor pathophysiology in the lung microcirculation.
我们基于皮下植入Lewis肺癌的小鼠建立了肺转移实验模型，并在体内观察了肺内自发转移肿瘤的微循环。用一个小型手工制作的吸引环固定小鼠肺部，以停止心脏和呼吸运动。使用荧光显微镜，通过测量微血管直径和血流速度（红细胞速度），比较同一肺叶内的肿瘤微循环和正常肺微循环。在正常微循环中，微血管直径和红细胞速度的平均值分别为10.4±2.7微米和188±63微米/秒。在肿瘤微循环中，相应的平均值分别为10.6±3.3微米和105±40微米/秒。正常微循环中的红细胞速度显著高于肿瘤微循环（P<0.001）。正常微循环和肿瘤微循环的计算剪切率分别为73.4±23.4（/秒）和41.2±16.1（/秒）。肿瘤微循环的剪切率明显慢于正常微循环（P<0.001）。我们证明了观察和测量肺内肿瘤微循环的可行性，并证实这些生理数据与其他人报道的脑或肝脏中的数据相符。该模型可能有助于研究肺微循环中转移性肿瘤的病理生理学。
