Lehr H A, Leunig M, Menger M D, Nolte D, Messmer K
Institute for Surgical Research, Klinikum Grosshadern, University of Munich, Germany.
Am J Pathol. 1993 Oct;143(4):1055-62.
For many years, observation chambers, implanted in various animal species and in man, have been used for intravital microscopy of the microcirculation in granulation tissue, preformed tissue, or of the microvascularization of tissue implants. We describe herein a skinfold chamber model for the intravital microscopic investigation of striated skin muscle in immunoincompetent, nude mice over a minimum period of 2 weeks. Using fluorescent markers for contrast enhancement of plasma (fluorescein isothiocyanate-dextran) and leukocytes (acridine orange), the presented model allows the quantitative analysis of 1) the microhemodynamic parameters microvessel diameter and red blood cell velocity in arterioles (16 to 50 mu diameter), capillaries (4 to 9 mu diameter), and post-capillary venules (19 to 60 mu diameter), 2) leukocyte/endothelium interaction in these vessel segments, 3) functional capillary density and intercapillary distance, and 4) endothelial cell integrity. These parameters can be assessed in the microcirculation of the striated muscle tissue under normal or pathological conditions, as well as in the microcirculation of transplanted xenogeneic (human) neoplastic and non-neoplastic tissue grafts.
多年来，植入各种动物物种和人体的观察室一直用于肉芽组织、预制组织中微循环的活体显微镜检查，或组织植入物的微血管形成研究。我们在此描述一种用于免疫缺陷裸鼠横纹皮肤肌活体显微镜检查的皮褶室模型，观察期至少为2周。使用荧光标记物增强血浆（异硫氰酸荧光素 - 葡聚糖）和白细胞（吖啶橙）的对比度，该模型能够对以下内容进行定量分析：1）小动脉（直径16至50微米）、毛细血管（直径4至9微米）和毛细血管后微静脉（直径19至60微米）中的微血管动力学参数微血管直径和红细胞速度；2）这些血管段中的白细胞/内皮细胞相互作用；3）功能性毛细血管密度和毛细血管间距；4）内皮细胞完整性。这些参数可在正常或病理条件下的横纹肌组织微循环中进行评估，也可在移植的异种（人）肿瘤和非肿瘤组织移植物的微循环中进行评估。
